Typical Testing Data/Standard Curve (for reference only)
Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit | ERK2 elisa kit
Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ERK2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ERK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ERK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ERK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]
Uniprot Description
ERK2: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.
Protein type: CMGC group; EC 2.7.11.24; ERK subfamily; Kinase, protein; MAPK family; MAPK/ERK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: axon; caveola; cytoplasm; cytoskeleton; cytosol; dendrite cytoplasm; early endosome; extracellular exosome; focal adhesion; Golgi apparatus; late endosome; microtubule organizing center; mitochondrion; nucleoplasm; nucleus; perikaryon; plasma membrane; protein complex; pseudopodium
Molecular Function: ATP binding; identical protein binding; kinase activity; MAP kinase activity; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; phosphatase binding; phosphotyrosine binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; RNA polymerase II carboxy-terminal domain kinase activity; transcription factor binding
Biological Process: activation of MAPK activity; apoptosis; B cell receptor signaling pathway; Bergmann glial cell differentiation; caveolin-mediated endocytosis; cell surface receptor signaling pathway; cellular response to amino acid starvation; cellular response to cadmium ion; cellular response to DNA damage stimulus; cellular response to organic substance; cellular response to reactive oxygen species; cytosine metabolic process; diadenosine tetraphosphate biosynthetic process; ERBB signaling pathway; ERK1 and ERK2 cascade; heart development; lipopolysaccharide-mediated signaling pathway; mammary gland epithelial cell proliferation; MAPK cascade; negative regulation of cell differentiation; neural crest cell development; organ morphogenesis; outer ear morphogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of gene expression; positive regulation of peptidyl-threonine phosphorylation; positive regulation of protein import into nucleus, translocation; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; positive regulation of transcription, DNA-templated; positive regulation of translation; protein amino acid phosphorylation; regulation of cellular pH; regulation of cytoskeleton organization; regulation of early endosome to late endosome transport; regulation of Golgi inheritance; regulation of ossification; regulation of protein stability; regulation of stress-activated MAPK cascade; response to estrogen; response to exogenous dsRNA; response to lipopolysaccharide; response to nicotine; response to toxin; sensory perception of pain; signal transduction; stress-activated MAPK cascade; T cell receptor signaling pathway; thymus development; thyroid gland development
Research Articles on ERK2
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Product Notes
The Rat ERK2 mapk1 (Catalog #AAA456855) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456855 ELISA Kit recognizes Rat ERK2. It is sometimes possible for the material contained within the vial of "Extracellular Signal Regulated Kinase 2 (ERK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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