Rat Extracellular Signal Regulated Kinase 2 (ERK2) RTU ELISA Kit | ERK2 rtu elisa kit

Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit

Cat. Num. AAA459111

• Gene Names: Mapk1; ERT1; Erk2; ERK-2; p42-MAPK
• Reactivity: Rat
• Synonyms: Extracellular Signal Regulated Kinase 2 (ERK2); Rat Extracellular Signal Regulated Kinase 2 (ERK2) ELISA Kit; MAPK1; P38; P40; ERK2; ERT1; MAPK2; P42MAPK; P42-MAPK; PRKM1; PRKM2; P41; P41mapk; P41-mapk; Mitogen-Activated Protein Kinase 1; Mitogen-activated protein kinase 2; ERK2 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 358

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERK2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ERK2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ERK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ERK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ERK2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16758698
• NCBI GeneID: 116590
• NCBI Accession #: NP_446294.1
• NCBI GenBank Nucleotide #: NM_053842.2
• UniProt Accession #: P63086; P27703
• Molecular Weight: 41,276 Da
• NCBI Official Full Name: mitogen-activated protein kinase 1
• NCBI Official Synonym Full Names: mitogen activated protein kinase 1
• NCBI Official Symbol: Mapk1
• NCBI Official Synonym Symbols: ERT1; Erk2; ERK-2; p42-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase 1
• UniProt Gene Name: Mapk1
• UniProt Synonym Gene Names: Erk2; Mapk; Prkm1; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2

NCBI Description

kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation (). Phosphorylates CDK2AP2 (PubMed:12944431).