IL-24 active protein

Human IL-24 Protein, His Tag

Cat. Num. AAA9718713

• Gene Names: IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B
• Purity: >94% as determined by SDS-PAGE
• Synonyms: IL-24; Human IL-24 Protein; His Tag; Recombinant Human IL24 Protein; His Tag (Interleukin-24) expressed in Human Cells; C49A; FISP; IL10B; MDA7; MOB5; ST16; Interleukin-24; IL24; IL-24 active protein

• Host: Human Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from human IL24 isoform 3 (NP_001172085.1) (Met 1-Leu 207) was fused with a polyhistidine tag at the C-terminus.
• Sequence Length: 207

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The secreted recombinant human IL24 consists of 166 amino acids and has a predicted molecular mass of 19.5 kDa. As a result of different glycosylation, the apparent molecular mass of rhIL24 is approximately 35 kDa in SDS-PAGE under reducing conditions.
• Activity: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL-20 Ralpha and human IL-20 Rbeta. The EC50 for this effect is typically 0.05-0.25 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL24 protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL-24 protein, His Tag.)

Related Product Information for IL-24 active protein

Interleukin-24 (IL-24) also known as Melanoma differentiation-associated gene 7 protein (MDA-7) is a member of the IL10 family of cytokines. IL-24/MDA-7/IL24 can induce apoptosis selectively in various cancer cells. Overexpression of IL-24 leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Human IL-24/MDA-7/IL24 is secreted by activated peripheral blood mononuclear cells and is the ligand for two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Northern blot analysis revealed IL-24/MDA-7/IL24 expression in human tissues associated with the immune system such as spleen, thymus, peripheral blood leukocytes and normal melanocytes. IL-24/MDA-7/IL24 binding to either its endogenous receptors on human keratinocytes or to ectopically expressed receptors on baby hamster kidney cells leads to activation of the signal transducers and activators of transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 297632401
• NCBI GeneID: 11009
• NCBI Accession #: NP_001172085.1
• NCBI GenBank Nucleotide #: NP_001172085.1
• UniProt Accession #: Q13007
• Molecular Weight: 19.5 kDa
• NCBI Official Full Name: interleukin-24 isoform 3
• NCBI Official Synonym Full Names: interleukin 24
• NCBI Official Symbol: IL24
• NCBI Official Synonym Symbols: C49A; FISP; MDA7; MOB5; ST16; IL10B
• NCBI Protein Information: interleukin-24

NCBI Description

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]