Human MutL Homolog 1 (MLH1) ELISA Kit | MLH1 elisa kit

Human MutL Homolog 1 (MLH1) ELISA Kit

Cat. Num. AAA452937

• Gene Names: MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• Reactivity: Human
• Synonyms: MutL Homolog 1 (MLH1); Human MutL Homolog 1 (MLH1) ELISA Kit; COCA2; FCC2; HNPCC; HNPCC2; hMLH1; DNA mismatch repair protein Mlh1; MLH1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MLH1. No significant cross-reactivity or interference between MLH1 and analogues was observed.
• Sequence Length: 658

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MLH1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MLH1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MLH1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MLH1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MLH1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MLH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MLH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MLH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557757
• NCBI GeneID: 4292
• NCBI Accession #: NP_000240.1
• NCBI GenBank Nucleotide #: NM_000249.3
• UniProt Accession #: P40692; B4DI13; B4DQ11; E9PCU2
• Molecular Weight: 73,837 Da
• NCBI Official Full Name: DNA mismatch repair protein Mlh1 isoform 1
• NCBI Official Synonym Full Names: mutL homolog 1
• NCBI Official Symbol: MLH1
• NCBI Official Synonym Symbols: FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• NCBI Protein Information: DNA mismatch repair protein Mlh1
• UniProt Protein Name: DNA mismatch repair protein Mlh1
• Protein Family: Putative MutL protein
• UniProt Gene Name: MLH1
• UniProt Synonym Gene Names: COCA2

NCBI Description

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009]

Uniprot Description

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.