Human MutL Homolog 1 (MLH1) ELISA Kit | MLH1 elisa kit

Human MutL Homolog 1 (MLH1) ELISA Kit

Cat. Num. AAA2706758

• Gene Names: MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• Reactivity: Human
• Synonyms: MutL Homolog 1 (MLH1); Human MutL Homolog 1 (MLH1) ELISA Kit; COCA2; FCC2; HNPCC; HNPCC2; hMLH1; DNA mismatch repair protein Mlh1; MLH1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MutL Homolog 1 (MLH1).; No significant cross-reactivity or interference between MutL Homolog 1 (MLH1) and analogues was observed.
• Sequence Length: 658

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MutL Homolog 1 (MLH1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MutL Homolog 1 (MLH1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MLH1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MutL Homolog 1 (MLH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MutL Homolog 1 (MLH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MutL Homolog 1 (MLH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MutL Homolog 1 (MLH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62897179
• NCBI GeneID: 4292
• UniProt Accession #: P40692; B4DI13; B4DQ11; E9PCU2
• Molecular Weight: 73,837 Da
• NCBI Official Full Name: MutL protein homolog 1 variant, partial
• NCBI Official Synonym Full Names: mutL homolog 1
• NCBI Official Symbol: MLH1
• NCBI Official Synonym Symbols: FCC2; COCA2; HNPCC; hMLH1; HNPCC2
• NCBI Protein Information: DNA mismatch repair protein Mlh1
• UniProt Protein Name: DNA mismatch repair protein Mlh1
• Protein Family: Putative MutL protein
• UniProt Gene Name: MLH1
• UniProt Synonym Gene Names: COCA2

NCBI Description

The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]

Uniprot Description

Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.