Human Lactamase Beta (LACTb) ELISA Kit | LACTb elisa kit

Human Lactamase Beta (LACTb) ELISA Kit

Cat. Num. AAA452035

• Gene Names: LACTB; G24; MRPL56
• Reactivity: Human
• Synonyms: Lactamase Beta (LACTb); Human Lactamase Beta (LACTb) ELISA Kit; G24; MRPL56; Mitochondrial Ribosomal Protein L56; Serine beta-lactamase-like protein LACTB; mitochondrial; LACTb elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LACTb. No significant cross-reactivity or interference between LACTb and analogues was observed.
• Sequence Length: 332

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.093ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LACTb were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LACTb were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LACTb elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LACTb in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LACTb. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LACTb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LACTb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 568384811
• NCBI GeneID: 114294
• NCBI Accession #: NP_001275514.1
• NCBI GenBank Nucleotide #: NM_001288585.1
• UniProt Accession #: P83111; P83096
• Molecular Weight: 41,239 Da
• NCBI Official Full Name: serine beta-lactamase-like protein LACTB, mitochondrial isoform c
• NCBI Official Synonym Full Names: lactamase beta
• NCBI Official Symbol: LACTB
• NCBI Official Synonym Symbols: G24; MRPL56
• NCBI Protein Information: serine beta-lactamase-like protein LACTB, mitochondrial
• UniProt Protein Name: Serine beta-lactamase-like protein LACTB, mitochondrial
• Protein Family: Serine beta-lactamase-like protein
• UniProt Gene Name: LACTB
• UniProt Synonym Gene Names: MRPL56

NCBI Description

This gene encodes a mitochondrially-localized protein that has sequence similarity to prokaryotic beta-lactamases. Many of the residues responsible for beta-lactamase activity are not conserved in this protein, suggesting it may have a different enzymatic function. Increased expression of the related mouse gene was found to be associated with obesity. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Dec 2013]