Human Filaggrin (FLG) ELISA Kit | FLG elisa kit

Human Filaggrin (FLG) ELISA Kit

Cat. Num. AAA451612

• Gene Names: FLG; ATOD2
• Reactivity: Human
• Synonyms: Filaggrin (FLG); Human Filaggrin (FLG) ELISA Kit; Profilaggrin; FLG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FLG. No significant cross-reactivity or interference between FLG and analogues was observed.
• Sequence Length: 4061

• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FLG were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FLG were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLG elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FLG in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FLG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FLG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FLG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FLG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 60097902
• NCBI GeneID: 2312
• NCBI Accession #: NP_002007.1
• NCBI GenBank Nucleotide #: NM_002016.1
• UniProt Accession #: P20930; Q01720; Q5T583; Q9UC71
• Molecular Weight: 435,170 Da
• NCBI Official Full Name: filaggrin
• NCBI Official Synonym Full Names: filaggrin
• NCBI Official Symbol: FLG
• NCBI Official Synonym Symbols: ATOD2
• NCBI Protein Information: filaggrin
• UniProt Protein Name: Filaggrin
• Protein Family: Filaggrin
• UniProt Gene Name: FLG

NCBI Description

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

Uniprot Description

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.