Horse Matrix Metalloproteinase 13 (MMP13) RTU ELISA Kit | MMP13 rtu elisa kit

Equine Matrix Metalloproteinase 13 (MMP13) ELISA Kit

Cat. Num. AAA4501066

• Reactivity: Horse
• Synonyms: Matrix Metalloproteinase 13 (MMP13); Equine Matrix Metalloproteinase 13 (MMP13) ELISA Kit; CLG3; Collagenase 3; MMP13 rtu elisa kit

• Reactivity: Horse
• Sequence Length: 472

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 6.9pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP13 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126352506
• NCBI GeneID: 100009711
• NCBI Accession #: NP_001075273.1
• NCBI GenBank Nucleotide #: NM_001081804.1
• UniProt Accession #: O18927
• Molecular Weight: 54,226 Da
• NCBI Official Full Name: collagenase 3
• NCBI Official Synonym Full Names: matrix metallopeptidase 13
• NCBI Official Symbol: MMP13
• NCBI Protein Information: collagenase 3
• UniProt Protein Name: Collagenase 3
• Protein Family: Collagenase
• UniProt Gene Name: MMP13
• UniProt Synonym Gene Names: MMP-13

Uniprot Description

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().