Rabbit anti-Human RBM42 Polyclonal Antibody | anti-RBM42 antibody

RBM42, CT (RBM42, RNA-binding protein 42, RNA-binding motif protein 42) (PE)

Cat. Num. AAA6340805

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RBM42; Polyclonal Antibody; CT (RBM42; RNA-binding protein 42; RNA-binding motif protein 42) (PE); RNA-binding motif protein 42; anti-RBM42 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RBM42 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RBM42 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-422 amino acids from the C-terminal region of human RBM42.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RBM42 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RBM42 antibody detected the RBM42 protein (arrow).)

Immunohistochemistry (IHC)

(RBM42 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RBM42 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RBM42 antibody

RBM42 binds (via the RRM domain) to the 3'-untranslated region (UTR) of CDKN1A mRNA (By similarity).

Product Categories/Family for anti-RBM42 antibody

Antibodies; Binding Proteins

References

Fukuda, T., et al. Genes Cells 14(2):113-128(2009). Lim, J., et al. Cell 125(4):801-814(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 21359951
• NCBI GeneID: 79171
• NCBI Accession #: NP_077297.2
• NCBI GenBank Nucleotide #: NM_024321.3
• UniProt Accession #: Q9BTD8; O00320; Q8N5R7; Q9BU66
• Molecular Weight: 50,414 Da
• NCBI Official Full Name: RNA-binding protein 42
• NCBI Official Synonym Full Names: RNA binding motif protein 42
• NCBI Official Symbol: RBM42
• NCBI Protein Information: RNA-binding protein 42; RNA-binding motif protein 42
• UniProt Protein Name: RNA-binding protein 42
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM42
• UniProt Entry Name: RBM42_HUMAN

Uniprot Description

Function: Binds (via the RRM domain) to the 3'-untranslated region (UTR) of CDKN1A mRNA

By similarity.

Subunit structure: Interacts with HNRNPK

By similarity.

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity. Note: Upon stress response, localizes with HNRNPK in cytoplasmic aggregates of stalled translational preinitiation complexes called stress granules

By similarity.

Sequence similarities: Belongs to the RRM RBM42 family.Contains 1 RRM (RNA recognition motif) domain.

Sequence caution: The sequence AAB57629.1 differs from that shown. Reason: Erroneous gene model prediction.