Rabbit p21 Polyclonal Antibody | anti-p21 antibody

p21 (C19) Antibody

Cat. Num. AAA440016

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: p21; Polyclonal Antibody; p21 (C19) Antibody; anti-p21 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p21 (C19) DB016 reacts with p21(also designated WAF1/Cip1) of mouse, rat, and human origin.
• Form/Format: PBS containing 0.1% sodium azide and 0.2% gelatin.
• Concentration: 200 ug/ml (varies by lot)
• Sequence Length: 164

• Applicable Applications for anti-p21 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Western blotting starting dilution: 1:200.
• Origin: p21 (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human p21.
• Competitition Studies: A blocking peptide is also available, MBS443016, for use in competition studies. Each vial contains 100 ug of peptide in 0.5mL PBS with 0.1% sodium azide and 100 ug BSA.
• Preparation and Storage: Store this product at 4°C, do not freeze.; ; The product is stable for one year from the date of shipment.

Related Product Information for anti-p21 antibody

The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

References

1.)Blank M, Lerenthal Y, Mittelman L, Shiloh Y. 2006. Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage. J Cell Biol. Jul 17;174(2):195-206.2.) Kwak YE, Jeon NK, Kim J, and Lee EJ. 2006. The mechanism of Cell Growth Inhibition and Apoptosis by Cyclooxygenase-2 Inhibitor in Oral Squamous Carcinoma Cells. Cancer Prev Res Vol.11, No.2, 89-98.3.) Kim TH, Oh S, Kim SS. 2005. Recombinant human prothrombin kringle-2 induces bovine capillary endothelial cell cycle arrest at G0-G1 phase through inhibition of cyclin D1/CDK4 complex: modulation of reactive oxygen species generation and up-regulation of cyclin-dependent kinase inhibitors. Angiogenesis. 8(4):307-14.

1. Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ. 1993. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75(4):805-816.2. Gartel AL, Serfas MS, Tyner AL. 1996. p21 - -negative regulator of the cell cycle. Proc Soc Exp Biol Med 213(2):138-149.3. Moller MB. 2000. p27 in cell cycle control and cancer. Leuk Lymphoma 39(1-2):19-27.4. Sgambato A, Cittadini A, Faraglia B, Weinstein IB. 2000. Multiple functions of p27(Kip1) and its alterations in tumor cells: a review. J Cell Physiol 183(1):18-27.5. Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R, Beach D. 1993. p21 is a universal inhibitor of cyclin kinases. Nature 366(6456):634.6. Gorospe M, Wang X, Holbrook NJ. 1999. Functional role of p21 during the cellular response to stress. Gene Expr 7(4-6):377-385.7. Slingerland J, Pagano M. 2000. Regulation of the cdk inhibitor p27 and its deregulation in cancer. J Cell Physiol 183(1):10-17.8. Toyoshima H, Hunter T. 1994. p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell 78(1):67-74.9. Shapiro GI, Edwards CD, Rollins BJ. 2000. The physiology of p16(INK4A)-mediated G1 proliferative arrest. Cell Biochem Biophys 33(2):189-197.10. Liggett WH Jr, Sidransky D. 1998. Role of the p16 tumor suppressor gene in cancer. J Clin Oncol 16(3):1197-1206.

NCBI and Uniprot Product Information

• NCBI GI #: 453135
• NCBI GeneID: 1026
• UniProt Accession #: P38936; Q14010; Q6FI05; Q9BUT4
• NCBI Official Full Name: p21
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDK-interaction protein 1; DNA synthesis inhibitor; melanoma differentiation associated protein 6; wild-type p53-activated fragment 1
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• Protein Family: P21 prophage-derived protein
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6
• UniProt Entry Name: CDN1A_HUMAN

NCBI Description

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Inhibitor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; cyclin binding; cyclin-dependent protein kinase activating kinase activity; metal ion binding; ubiquitin protein ligase binding; protein complex binding

Biological Process: nerve growth factor receptor signaling pathway; response to toxin; response to arsenic; protein amino acid phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of B cell proliferation; G2/M transition of mitotic cell cycle; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; response to corticosterone stimulus; response to drug; epidermal growth factor receptor signaling pathway; organ regeneration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of cyclin-dependent protein kinase activity; response to organic nitrogen; positive regulation of programmed cell death; response to hyperoxia; positive regulation of protein kinase activity; Ras protein signal transduction; cellular response to extracellular stimulus; innate immune response; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of cell growth; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle