CD10 Active Protein | MME active protein

CD10 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122618

• Gene Names: Mme; NEP; SFE; CD10; CALLA; C85356; 6030454K05Rik
• Purity: >98% as determined by SDS-PAGE
• Synonyms: CD10; CD10 Protein; Mouse; Recombinant (His Tag); Mouse CD10/Neprilysin/MME Protein (His Tag); 6030454K05Rik Protein; C85356 Protein; CALLA Protein; NEP Protein; SFE Protein; membrane metallo-endopeptidase; MME active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Tyr52-Trp750

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Tyr 52-Trp 750) of mouse Mme (NP_032630.2) was fused with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH The specific activity is >3000pmoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MME active protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. The cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had the function to degrade some small secreted peptides such as the amyloid beta-peptide. It exists as a membrane-bound protein and has a high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12Dogan, et al. (2000) CD10 and BCL-6 Expression in Paraffin Sections of Normal Lymphoid Tissue and B-Cell Lymphomas. American Journal of Surgical Pathology. 24(6): 846-52.

NCBI and Uniprot Product Information

• NCBI GI #: 31543255
• NCBI GeneID: 17380
• NCBI Accession #: NP_032630.2
• NCBI GenBank Nucleotide #: NM_008604.3
• UniProt Accession #: Q61391; Q6NXX5; Q8K251
• Molecular Weight: 85,702 Da
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metallo endopeptidase
• NCBI Official Symbol: Mme
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA; C85356; 6030454K05Rik
• NCBI Protein Information: neprilysin; enkephalinase; atriopeptidase; skin fibroblast elastase; neutral endopeptidase 24.11; common acute lymphoblastic leukemia antigen
• UniProt Protein Name: Neprilysin
• Protein Family: CD109 antigen
• UniProt Gene Name: Mme
• UniProt Synonym Gene Names: NEP; Neutral endopeptidase; SFE
• UniProt Entry Name: NEP_MOUSE

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: Membrane protein, integral; Protease; EC 3.4.24.11

Cellular Component: synaptic vesicle; focal adhesion; membrane; axon; cytoplasm; dendrite; plasma membrane; integral to membrane; synapse; brush border

Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metalloendopeptidase activity; metal ion binding; endopeptidase activity; exopeptidase activity; peptide binding

Biological Process: peptide metabolic process; beta-amyloid metabolic process; creatinine metabolic process; sensory perception of pain; proteolysis