Human Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit | NEIL3 elisa kit

Human Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit

Cat. Num. AAA457091

• Gene Names: NEIL3; FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• Reactivity: Human
• Synonyms: Nei Endonuclease VIII Like Protein 3 (NEIL3); Human Nei Endonuclease VIII Like Protein 3 (NEIL3) ELISA Kit; FPG2; hFPG2; hNEI3; Endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; Nei-like protein 3; NEIL3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NEIL3. No significant cross-reactivity or interference between NEIL3 and analogues was observed.
• Sequence Length: 605

• Samples: tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: <34.55pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEIL3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEIL3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NEIL3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NEIL3 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEIL3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NEIL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEIL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEIL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 157388969
• NCBI GeneID: 55247
• NCBI Accession #: NP_060718.2
• NCBI GenBank Nucleotide #: NM_018248.2
• UniProt Accession #: Q8TAT5; Q2PPJ3; Q8NG51; Q9NV95
• Molecular Weight: 67,769 Da
• NCBI Official Full Name: endonuclease 8-like 3
• NCBI Official Synonym Full Names: nei like DNA glycosylase 3
• NCBI Official Symbol: NEIL3
• NCBI Official Synonym Symbols: FGP2; FPG2; NEI3; ZGRF3; hFPG2; hNEI3
• NCBI Protein Information: endonuclease 8-like 3
• UniProt Protein Name: Endonuclease 8-like 3
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL3

NCBI Description

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]

Uniprot Description

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.