Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed Raji cells. ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Mouse anti-Human ZMYM3 Monoclonal Antibody | anti-ZMYM3 antibody
ZMYM3 Mouse Monoclonal Antibody [Clone PCRP-ZMYM3-2F10]
Gene Names
ZMYM3; MYM; XFIM; ZNF261; DXS6673E; ZNF198L2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
ZMYM3; Monoclonal Antibody; ZMYM3 Mouse Monoclonal Antibody [Clone PCRP-ZMYM3-2F10]; DXS6673E; KIAA0385; MYM; XFIM; ZFP261; Zinc finger MYM type 3; Zinc finger MYM type protein 3; Zinc finger MYM-type protein 3; Zinc finger protein 261; ZNF198L2; ZNF261; anti-ZMYM3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG2a
Clone Number
PCRP-ZMYM3-2F10
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-ZMYM3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F)
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
Immunogen
Recombinant full-length human ZMYM3 protein
Cellular Localization
Nucleus
Positive Control
HeLa or Raji cells. Ubiquitious nuclear expression.
Hu-chromosome Location
Xq13.1
Unigene
522684
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed Raji cells. ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of PFA-fixed Raji cells. ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )
Related Product Information for anti-ZMYM3 antibody
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZMYM3 (zinc finger MYM-type protein 3), also known as ZNF261 (zinc finger protein 261), XFIM, DXS6673E or MYM, is a 1,370 amino acid nuclear protein that contains nine MYM-type zinc fingers. Expressed in a variety of tissues, including heart, muscle and brain, ZMYM3 is thought to function as part of a histone deacetylase-containing complex that contains other proteins, such as HDAC1 and HDAC2, and may play a role in gene silencing through the modification of chromatin structure. Defects in the gene encoding ZMYM3 that lead to chromosomal translocations may be a cause of X-linked mental retardation. Two isoforms of ZMYM3 exist due to alternative splicing events.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
52,485 Da
NCBI Official Full Name
zinc finger, MYM-type 3, isoform CRA_a
NCBI Official Synonym Full Names
zinc finger, MYM-type 3
NCBI Official Symbol
ZMYM3
NCBI Official Synonym Symbols
MYM; XFIM; ZNF261; DXS6673E; ZNF198L2
NCBI Protein Information
zinc finger MYM-type protein 3; zinc finger protein 261
UniProt Protein Name
Zinc finger MYM-type protein 3
Protein Family
UniProt Gene Name
ZMYM3
UniProt Synonym Gene Names
DXS6673E; KIAA0385; ZNF261
UniProt Entry Name
ZMYM3_HUMAN
NCBI Description
This gene is located on the X chromosome and is subject to X inactivation. It is highly conserved in vertebrates and most abundantly expressed in the brain. The encoded protein is a component of histone deacetylase-containing multiprotein complexes that function through modifying chromatin structure to keep genes silent. A chromosomal translocation (X;13) involving this gene is associated with X-linked mental retardation. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2010]
Uniprot Description
ZNF261: Plays a role in the regulation of cell morphology and cytoskeletal organization. A chromosomal aberration involving ZMYM3 may be a cause of X-linked mental retardation in Xq13.1. Translocation t(X;13)(q13.1;?). 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: nucleus
Molecular Function: DNA binding; zinc ion binding
Biological Process: multicellular organismal development; regulation of cell morphogenesis; cytoskeleton organization and biogenesis
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Product Notes
The ZMYM3 zmym3 (Catalog #AAA4382360) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZMYM3 Mouse Monoclonal Antibody [Clone PCRP-ZMYM3-2F10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZMYM3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F). FC/FACS: 1-2ug/million cells IF: 1-2ug/ml WB: 1-2ug/ml IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ZMYM3 zmym3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZMYM3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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