Western Blot (WB)
(Western blot -Actin 5C, isoform B Mouse mAb for the adult stage whole body of ant All lanes: Actin 5C , isoform B Mouse mAb for the adult stage whole body of ant at different dilutionLane 1: 1/1000 dilutionLane 2: 1/2000 dilutionLane3: 1/5000 dilutionLysates/proteins at 20 ug per laneSecondary:Goat Anti -Mouse IgG-HRP , 5% skimmilk conjugated at 1/10000 dilutionpredicted band size :42 kDaObserved band size: 42 kDaBlocking/Dilution buffer: 1xPBS )
Mouse Actin 5C Monoclonal Antibody | anti-Act5C antibody
Actin 5C, isoform B Mouse mAb for Ant
Gene Names
Act5C; A; A4V404_DROME; Ac5C; act; Act; ACT; act 5C; Act-5C; ACT1_DROME; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; ArpA; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; chrX:5748184..5748304; cyt5C; DmelCG4027; l
Reactivity
Camponotus floridanus
Applications
Western Blot
Synonyms
Actin 5C; Monoclonal Antibody; isoform B Mouse mAb for Ant; Act5C Mouse mAb; anti-Act5C antibody
Host
Mouse
Reactivity
Camponotus floridanus
Clonality
Monoclonal
Isotype
IgG
Sequence Length
376
Applicable Applications for anti-Act5C antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:5000
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Source Note
This monoclonal antibody is produced by immunizing mice with a polypeptide corresponding to actin 5C, isoform B protein.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Western Blot (WB)
(Western blot -Actin 5C, isoform B Mouse mAb for the adult stage whole body of ant All lanes: Actin 5C , isoform B Mouse mAb for the adult stage whole body of ant at different dilutionLane 1: 1/1000 dilutionLane 2: 1/2000 dilutionLane3: 1/5000 dilutionLysates/proteins at 20 ug per laneSecondary:Goat Anti -Mouse IgG-HRP , 5% skimmilk conjugated at 1/10000 dilutionpredicted band size :42 kDaObserved band size: 42 kDaBlocking/Dilution buffer: 1xPBS )
Western Blot (WB)
(Western blot -Actin 5C, isoform B Mouse mAb for the adult stage whole body of ant All lanes: Actin 5C , isoform B Mouse mAb for the adult stage whole body of ant at different dilutionLane 1: 1/1000 dilutionLane 2: 1/2000 dilutionLane3: 1/5000 dilutionLysates/proteins at 20 ug per laneSecondary:Goat Anti -Mouse IgG-HRP , 5% skimmilk conjugated at 1/10000 dilutionpredicted band size :42 kDaObserved band size: 42 kDaBlocking/Dilution buffer: 1xPBS )
Related Product Information for anti-Act5C antibody
The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-Act5C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 42kDa
NCBI Official Full Name
actin 5C, isoform E
NCBI Official Synonym Full Names
Actin 5C
NCBI Official Symbol
Act5C
NCBI Official Synonym Symbols
A; A4V404_DROME; Ac5C; act; Act; ACT; act 5C; Act-5C; ACT1_DROME; Act5; act5C; Act5c; ACT5C; actin; Actin; Actin/BAP47; actin5C; Actin5C; anon-EST:fe2D2; ArpA; Bap47; BAP47; beta-actin; beta-actin/Bap47; CG4027; chrX:5748184..5748304; cyt5C; DmelCG4027; l
NCBI Protein Information
actin 5C
UniProt Protein Name
Actin-5C
Protein Family
UniProt Gene Name
Act5C
Uniprot Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Research Articles on Act5C
Similar Products
Product Notes
The Act5C act5c (Catalog #AAA435432) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin 5C, isoform B Mouse mAb for Ant reacts with Camponotus floridanus and may cross-react with other species as described in the data sheet. AAA Biotech's Actin 5C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:5000 IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. . Researchers should empirically determine the suitability of the Act5C act5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin 5C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
