Goat SOX10 Antibody | anti-SOX10 antibody

Goat Anti-SOX10 (aa204-217) Antibody

Cat. Num. AAA423861

• Gene Names: SOX10; DOM; WS4; PCWH; WS2E; WS4C
• Reactivity: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SOX10; Goat Anti-SOX10 (aa204-217) Antibody; SRY (sex determining region Y)-box 10; DOM; MGC15649; PCWH; WS2E; WS4; WS4C; OTTHUMP00000195094; OTTHUMP00000195097; SRY-related HMG-box gene 10; dominant megacolon; mouse; human homolog of; transcription factor SOX-10; anti-SOX10 antibody

• Host: Goat
• Reactivity: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 466

• Applicable Applications for anti-SOX10 antibody: Peptide ELISA (EIA), WB
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:4000; WB: Approx 50kDa band observed in Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Mouse NP_035567.1 and 50.0kDa according to Rat NP_062066.1). Recommended concentration: 0.3-0.5ug/ml. Primary incubation 1 hour at room temperature.
• Immunogen: Peptide with sequence C-QAHYKSAHLDHRHP, from the C or N Terminus of the protein sequence according to NP_008872.1.
• Peptide Sequence: QAHYKSAHLDHRHP
• Epitope: C or N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

References

Agnarsdóttir M, Sooman L, Bolander A, Strömberg S, Rexhepaj E, Bergqvist M, Ponten F, Gallagher W, Lennartsson J, Ekman S, Uhlen M, Hedstrand H.: SOX10 expression in superficial spreading and nodular malignant melanomas. Melanoma Res. 2010 Dec;20(6):468-78. PMID: 20890226

NCBI and Uniprot Product Information

• NCBI GI #: 5902104
• NCBI GeneID: 6663
• NCBI Accession #: NP_008872.1
• NCBI GenBank Nucleotide #: NP_008872.1
• UniProt Accession #: P56693
• NCBI Official Full Name: transcription factor SOX-10
• NCBI Official Synonym Full Names: SRY-box transcription factor 10
• NCBI Official Symbol: SOX10
• NCBI Official Synonym Symbols: DOM; WS4; PCWH; WS2E; WS4C
• NCBI Protein Information: transcription factor SOX-10
• UniProt Protein Name: Transcription factor SOX-10
• Protein Family: Transcription factor
• UniProt Gene Name: SOX10

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX10: Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E). WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C); also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH); also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.

Protein type: Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; transcription coactivator activity

Biological Process: anatomical structure morphogenesis; regulation of transcription from RNA polymerase II promoter

Disease: Peripheral Demyelinating Neuropathy, Central Dysmyelination, Waardenburg Syndrome, And Hirschsprung Disease; Waardenburg Syndrome, Type 2e; Waardenburg Syndrome, Type 4c