Rabbit anti-Human V-Jun Sarcoma Virus 17 Oncogene Homolog Polyclonal Antibody | anti-JUN antibody

Polyclonal Antibody to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)

Cat. Num. AAA2027191

• Gene Names: JUN; AP1; p39; AP-1; c-Jun
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: V-Jun Sarcoma Virus 17 Oncogene Homolog; Polyclonal Antibody; Polyclonal Antibody to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN); anti-JUN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against JUN. It has beenselected for its ability to recognize JUN in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DDALNASFLP SESGPYGYSN PKILKQSMTL NLADPVGSLK PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVNGA GMVAPAVASV AGGSGSGGFS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPAQ PQQQQQPPHH LPQQMPVQHP RLQALKEEPQ TVPEMPGETP PLSPIDMESQ ERIKAERKRM RN

• Applicable Applications for anti-JUN antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Fragment: JUN (Asp11~Asn262)
• Organism Species: Homo sapiens (Human)
• Cross Reactivity: Human
• Conjugate: No Conjugate
• Immunogen: Recombinant JUN (Asp11~Asn262) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2053582
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Human Placenta Tissue; Lane2: Human HepG2 Cells; Lane3: Human U-2 OS Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant JUN, Human.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Ovary tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 4758616
• NCBI GeneID: 3725
• NCBI Accession #: NP_002219.1
• NCBI GenBank Nucleotide #: NM_002228.3
• UniProt Accession #: P05412; Q6FHM7; Q96G93
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: transcription factor AP-1
• NCBI Official Synonym Full Names: Jun proto-oncogene, AP-1 transcription factor subunit
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; p39; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: Viral jun-transforming protein
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).