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Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Goat EPHB6 Antibody | anti-EPHB6 antibody
Goat Anti-EPHB6 Antibody
Reactivity
HumanExpected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
EPHB6; Goat Anti-EPHB6 Antibody; EPH receptor B6; HEP; MGC129910; MGC129911; ephrin receptor EphB6; anti-EPHB6 antibody
Host
Goat
Reactivity
Human
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Sequence Length
1021
Applicable Applications for anti-EPHB6 antibody
Peptide ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000
IHC: Paraffin embedded Human Small Intestine and Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
IF: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
FC/FACS: Flow cytometric analysis of HeLa cells.
IHC: Paraffin embedded Human Small Intestine and Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
IF: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
FC/FACS: Flow cytometric analysis of HeLa cells.
Peptide Sequence
C-EVIAHGKHTTSSD
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.75ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ephrin type-B receptor 6
Protein Family
Similar Products
Product Notes
The EPHB6 (Catalog #AAA423851) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-EPHB6 Antibody reacts with Human Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's EPHB6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Peptide ELISA: antibody detection limit dilution 1:32000 IHC: Paraffin embedded Human Small Intestine and Brain (Cerebellum). Recommended concentration: 3.75ug/ml. IF: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. FC/FACS: Flow cytometric analysis of HeLa cells. Researchers should empirically determine the suitability of the EPHB6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHB6, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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