Rabbit anti-Human EphB6 Polyclonal Antibody | anti-EPHB6 antibody

EphB6, CT (H990) (Ephrin Type-A Receptor 6, Tyrosine-protein Kinase Receptor EPH-6, HEP)

Cat. Num. AAA627780

• Gene Names: EPHB6; HEP; MGC129910; MGC129911
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: EphB6; Polyclonal Antibody; CT (H990) (Ephrin Type-A Receptor 6; Tyrosine-protein Kinase Receptor EPH-6; HEP); Anti -EphB6; anti-EPHB6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphB6.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-EPHB6 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from C-terminal region with histidine at postion 990 of human EphB6 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-EphB6 C-term Pab in Jurkat cell lysate. EphB6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-EPHB6 antibody

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.

Product Categories/Family for anti-EPHB6 antibody

Antibodies; Abs to Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 194097332
• NCBI GeneID: 2051
• NCBI Accession #: NP_004436.2
• NCBI GenBank Nucleotide #: NM_004445.3
• UniProt Accession #: O15197; Q2TB23; Q2TB24; A4D2I7; A8CDT5; D3DXD3
• Molecular Weight: 110,700 Da
• NCBI Official Full Name: ephrin type-B receptor 6
• NCBI Official Synonym Full Names: EPH receptor B6
• NCBI Official Symbol: EPHB6
• NCBI Official Synonym Symbols: HEP; MGC129910; MGC129911
• NCBI Protein Information: ephrin type-B receptor 6; OTTHUMP00000208518; tyrosine-protein kinase-defective receptor EPH-6
• UniProt Protein Name: Ephrin type-B receptor 6
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB6
• UniProt Entry Name: EPHB6_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ephrin receptor encoded by this gene lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands. [provided by RefSeq]

Uniprot Description

EphB6: Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Kinase, protein; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q33-q35

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytosol

Molecular Function: ephrin receptor activity; receptor activity; ATP binding

Biological Process: axon guidance; ephrin receptor signaling pathway; protein amino acid phosphorylation