Monkey Aquaporin 1, Colton Blood Group ELISA Kit | AQP1 elisa kit

Monkey Aquaporin 1, Colton Blood Group ELISA Kit

Cat. Num. AAA745232

• Gene Names: AQP1; CO; CHIP28; AQP-CHIP
• Reactivity: Monkey
• Synonyms: Aquaporin 1; Colton Blood Group; Monkey Aquaporin 1; Colton Blood Group ELISA Kit; AQP1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This AQP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey AQP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP1 elisa kit

Principle of the assay: AQP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AQP1 antibody and an AQP1-HRP conjugate. The assay sample and buffer are incubated together with AQP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP1 concentration since AQP1 from samples and AQP1-HRP conjugate compete for the anti-AQP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP1 from the sample, fewer sites are left to bind AQP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AQP1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 37694062
• NCBI GeneID: 358
• NCBI Accession #: NP_932766.1
• NCBI GenBank Nucleotide #: NM_198098.2
• Molecular Weight: 16,677 Da
• NCBI Official Full Name: aquaporin-1 isoform 1
• NCBI Official Synonym Full Names: aquaporin 1 (Colton blood group)
• NCBI Official Symbol: AQP1
• NCBI Official Synonym Symbols: CO; CHIP28; AQP-CHIP
• NCBI Protein Information: aquaporin-1; aquaporin-CHIP; urine water channel; channel-like integral membrane protein, 28-kDa; water channel protein for red blood cells and kidney proximal tubule; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group)
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

NCBI Description

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear membrane; brush border membrane; integral to plasma membrane; apical part of cell; basolateral plasma membrane; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; nerve terminal; nucleus; brush border; sarcolemma

Molecular Function: protein binding; ephrin receptor binding; potassium channel activity; water transporter activity; ammonium transmembrane transporter activity; intracellular cGMP activated cation channel activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; transmembrane transporter activity; nitric oxide transporter activity; water channel activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; carbon dioxide transport; water transport; cellular response to stress; negative regulation of caspase activity; sensory perception of pain; pancreatic juice secretion; positive regulation of saliva secretion; odontogenesis; positive regulation of fibroblast proliferation; hyperosmotic salinity response; nitric oxide transport; bicarbonate transport; multicellular organismal water homeostasis; cellular homeostasis; transmembrane transport; lipid digestion; potassium ion transport; lateral ventricle development; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; positive regulation of angiogenesis; renal water transport; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; renal water homeostasis; cell volume homeostasis; negative regulation of apoptosis

Disease: Blood Group--colton