Western Blot (WB)
((1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ICOS ligand/B7-H2 Polyclonal Antibody | anti-ICOSLG antibody
Goat anti-ICOS ligand/B7-H2 Antibody
Gene Names
ICOSLG; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ICOS ligand/B7-H2; Polyclonal Antibody; Goat anti-ICOS ligand/B7-H2 Antibody; ICOSLG; inducible T-cell co-stimulator ligand; B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; B7 homolog 2; B7 homologue 2; B7-like protein Gl50; B7-related protein 1; ICOS ligand; transmembrane protein B7-H2 ICOS ligand; ICOSLG antibody; inducible T-cell co-stimulator ligand antibody; B7-H2 antibody; B7H2 antibody; B7RP-1 antibody; B7RP1 antibody; CD275 antibody; GL50 antibody; ICOS-L antibody; ICOSL antibody; LICOS antibody; B7 homolog 2 antibody; B7 homologue 2 antibody; B7-like protein Gl50 antibody; B7-related protein 1 antibody; ICOS ligand antibody; transmembrane protein B7-H2 ICOS ligand antibody; ICOS ligand / B7-H2; anti-ICOSLG antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SQTGNDIGERDKIT
Sequence Length
302
Applicable Applications for anti-ICOSLG antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 33kDa band observed in Human Lymph Node lysates (calculated MW of 33.3kDa according to NP_056074.1). Recommended concentration: 1-3ug/ml.
Western Blot: Approx 33kDa band observed in Human Lymph Node lysates (calculated MW of 33.3kDa according to NP_056074.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-SQTGNDIGERDKIT, from the internal region of the protein sequence according to NP_056074.1; NP_001269979.1; NP_001269980.1; NP_001269981.1.
Epitope
Internal region
Note
This antibody is expected to recognize all reported isoforms (NP_056074.1; NP_001269979.1; NP_001269980.1; NP_001269981.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,120 Da
NCBI Official Full Name
ICOS ligand isoform a
NCBI Official Synonym Full Names
inducible T-cell co-stimulator ligand
NCBI Official Symbol
ICOSLG
NCBI Official Synonym Symbols
B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
NCBI Protein Information
ICOS ligand
UniProt Protein Name
ICOS ligand
Protein Family
UniProt Gene Name
ICOSLG
UniProt Synonym Gene Names
B7H2; B7RP1; ICOSL; KIAA0653; B7-H2; B7RP-1
UniProt Entry Name
ICOSL_HUMAN
Uniprot Description
ICOSLG: Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co- stimulating memory T-cell function. Belongs to the immunoglobulin superfamily. BTN/MOG family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: integral to membrane; plasma membrane
Molecular Function: receptor binding
Biological Process: adaptive immune response; B cell activation; defense response; hyperosmotic response; positive regulation of activated T cell proliferation; signal transduction; T cell activation; T cell costimulation
Research Articles on ICOSLG
Similar Products
Product Notes
The ICOSLG icoslg (Catalog #AAA423551) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ICOS ligand/B7-H2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ICOS ligand/B7-H2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 33kDa band observed in Human Lymph Node lysates (calculated MW of 33.3kDa according to NP_056074.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ICOSLG icoslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SQTGNDIGER DKIT. It is sometimes possible for the material contained within the vial of "ICOS ligand/B7-H2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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