Goat CBX7 Polyclonal Antibody | anti-CBX7 antibody

Goat anti-CBX7 (aa88-99) Antibody

Cat. Num. AAA423322

• Reactivity: Expected from sequence similarity: Human, Dog, Pig, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CBX7; Polyclonal Antibody; Goat anti-CBX7 (aa88-99) Antibody; chromobox homolog 7; chromobox protein homolog 7; CBX7 antibody; chromobox homolog 7 antibody; chromobox protein homolog 7 antibody; CBX7 (aa88-99); anti-CBX7 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DLRSSHKAKGKE
• Sequence Length: 251

• Applicable Applications for anti-CBX7 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Preliminary experiments gave an approx 23kDa band in lysates of cell lines A549, CACO2, MCF7 and MRC5 PD19 after 0. 01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.3kDa according to NP_783640.1. The 23kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-DLRSSHKAKGKE, from the internal region of the protein sequence according to NP_783640.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 28372505
• NCBI GeneID: 23492
• NCBI Accession #: NP_783640.1
• NCBI GenBank Nucleotide #: NM_175709.3
• Molecular Weight: 28,341 Da
• NCBI Official Full Name: chromobox protein homolog 7
• NCBI Official Synonym Full Names: chromobox 7
• NCBI Official Symbol: CBX7
• NCBI Protein Information: chromobox protein homolog 7
• UniProt Protein Name: Chromobox protein homolog 7
• Protein Family: Chromobox protein
• UniProt Gene Name: CBX7
• UniProt Entry Name: CBX7_HUMAN

Uniprot Description

CBX7: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Promotes histone H3 trimethylation at 'Lys-9' (H3K9me3). Binds to trimethylated lysine residues in histones, and possibly also other proteins. Regulator of cellular lifespan by maintaining the repression of CDKN2A, but not by inducing telomerase activity.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; heterochromatin; nuclear chromatin; nucleoplasm; nucleus; PcG protein complex

Molecular Function: chromatin binding; methylated histone residue binding; protein binding; single-stranded RNA binding

Biological Process: chromatin modification; negative regulation of transcription from RNA polymerase II promoter; response to drug; sebaceous gland development; transcription, DNA-dependent