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Testing Data (P-peptide-+)

TEBP Antibody | anti-TEBP antibody

TEBP (Phospho-Ser113) Antibody

Gene Names
PTGES3; P23; TEBP; cPGES
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
TEBP; TEBP (Phospho-Ser113) Antibody; EC 5.3.99.3; Hsp90 co-chaperone; P23; PGE synthase; cytosolic; PTGES3; Progesterone receptor complex p23; cPGES; anti-TEBP antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
TEBP (Phospho-Ser113) antibody detects endogenous levels of TEBP only when phosphorylated at serine 113.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
139
Applicable Applications for anti-TEBP antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human TEBP around the phosphorylation site of serine 113 (D-D-SP-D-E).
All Sites
Human: Ser113
Mouse: Ser113
Rat: Ser245
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TEBP (Phospho-Ser113) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 30mins), using TEBP (Phospho-Ser113) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TEBP (Phospho-Ser113) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 30mins), using TEBP (Phospho-Ser113) antibody.)
References
Hidenori Oguchi, Endocrinology, Apr 1998; 139: 1999. Takashi Kusakabe, Mol. Endocrinol., Aug 2006; 20: 1796-1809. K Mizuno, Mol. Cell. Biol., Oct 1991; 11: 4927-4933. S Kimura, Genes & Dev., Jan 1996; 10: 60-69.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,476 Da
NCBI Official Full Name
prostaglandin E synthase 3 isoform b
NCBI Official Synonym Full Names
prostaglandin E synthase 3
NCBI Official Symbol
PTGES3
NCBI Official Synonym Symbols
P23; TEBP; cPGES
NCBI Protein Information
prostaglandin E synthase 3
UniProt Protein Name
Prostaglandin E synthase 3
UniProt Gene Name
PTGES3
UniProt Synonym Gene Names
P23; TEBP; cPGES

NCBI Description

This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes. [provided by RefSeq, Feb 2016]

Uniprot Description

Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2) (PubMed:10922363). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes (PubMed:11274138, PubMed:12077419). Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway (PubMed:24711448).

Research Articles on TEBP

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Product Notes

The TEBP ptges3 (Catalog #AAA859775) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The TEBP (Phospho-Ser113) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TEBP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the TEBP ptges3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TEBP, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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