Western Blot (WB)
((0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Wfdc1 Polyclonal Antibody | anti-Wfdc1 antibody
Goat anti-Wfdc1 (mouse) Antibody
Gene Names
Wfdc1; ps20; 2310058A03Rik
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Wfdc1; Polyclonal Antibody; Goat anti-Wfdc1 (mouse) Antibody; WFDC1; WAP four-disulfide core domain 1; ps20; WAP four-disulfide core domain 1 homolog; WAP four-disulfide core domain protein 1; prostate stromal protein ps20; ps20 growth inhibitor; 2310058A03Rik; WFDC1 antibody; WAP four-disulfide core domain 1 antibody; ps20 antibody; WAP four-disulfide core domain 1 homolog antibody; WAP four-disulfide core domain protein 1 antibody; prostate stromal protein ps20 antibody; ps20 growth inhibitor antibody; 2310058A03Rik antibody; Wfdc1 (mouse); anti-Wfdc1 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RQRLHKEYPEGDSKN
Sequence Length
211
Applicable Applications for anti-Wfdc1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 19kDa band observed in Mouse Heart lysates (calculated MW of 23.1kDa according to NP_075884.1). Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 19kDa band observed in Mouse Heart lysates (calculated MW of 23.1kDa according to NP_075884.1). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-RQRLHKEYPEGDSKN, from the internal region (near N Terminus) of the protein sequence according to NP_075884.1.
Epitope
Internal region (near N Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,079 Da
NCBI Official Full Name
WAP four-disulfide core domain protein 1
NCBI Official Synonym Full Names
WAP four-disulfide core domain 1
NCBI Official Symbol
Wfdc1
NCBI Official Synonym Symbols
ps20; 2310058A03Rik
NCBI Protein Information
WAP four-disulfide core domain protein 1
UniProt Protein Name
WAP four-disulfide core domain protein 1
Protein Family
UniProt Gene Name
Wfdc1
UniProt Synonym Gene Names
Ps20
UniProt Entry Name
WFDC1_MOUSE
Uniprot Description
WFDC1: Has growth inhibitory activity.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: protease inhibitor activity; serine-type endopeptidase inhibitor activity
Biological Process: negative regulation of epithelial cell proliferation; negative regulation of inflammatory response; negative regulation of peptidase activity; regulation of cell growth
Research Articles on Wfdc1
Similar Products
Product Notes
The Wfdc1 wfdc1 (Catalog #AAA422848) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Wfdc1 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Wfdc1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 19kDa band observed in Mouse Heart lysates (calculated MW of 23.1kDa according to NP_075884.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the Wfdc1 wfdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RQRLHKEYPE GDSKN. It is sometimes possible for the material contained within the vial of "Wfdc1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
