Goat Cd28 Polyclonal Antibody | anti-Cd28 antibody

Goat anti-Cd28 (mouse) Antibody

Cat. Num. AAA422610

• Reactivity: Tested: Mouse; Expected from sequence similarity: Mouse
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Cd28; Polyclonal Antibody; Goat anti-Cd28 (mouse) Antibody; CD28 antigen; CH29-189K17.3; OTTMUSP00000002157; T-cell-specific surface glycoprotein CD28; Cd28 antibody; CD28 antigen antibody; CH29-189K17.3 antibody; OTTMUSP00000002157 antibody; T-cell-specific surface glycoprotein CD28 antibody; Cd28 (mouse); anti-Cd28 antibody

• Host: Goat
• Reactivity: Tested: Mouse; Expected from sequence similarity: Mouse
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KEKHLCHTQSSPK
• Sequence Length: 218

• Applicable Applications for anti-Cd28 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 40kDa band observed in Mouse Thymus lysates (calculated MW of 25.2kDa according to NP_031668.3). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence KEKHLCHTQSSPK, from the internal region of the protein sequence according to NP_031668.3.
• Epitope: Internal region
• Note: The immunizing peptide represents a part of the extracellular domain.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 161484597
• NCBI GeneID: 12487
• NCBI Accession #: NP_031668.3
• NCBI GenBank Nucleotide #: NM_007642.4
• Molecular Weight: 25,243 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28
• UniProt Entry Name: CD28_MOUSE

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: cell surface; external side of plasma membrane; immunological synapse; integral to membrane; lipid raft; membrane; plasma membrane

Molecular Function: protease binding; protein homodimerization activity; protein kinase binding; SH3/SH2 adaptor activity

Biological Process: immune response; negative thymic T cell selection; positive regulation of alpha-beta T cell proliferation; positive regulation of inflammatory response to antigenic stimulus; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of isotype switching to IgG isotypes; positive regulation of mitosis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of regulatory T cell differentiation; regulatory T cell differentiation; T cell activation; T cell costimulation; T cell receptor signaling pathway