Western Blot (WB)
((0.5ug/ml) staining of fetal Mouse kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SIM2 Polyclonal Antibody | anti-SIM2 antibody
Goat anti-SIM2 Antibody
Western Blot: Approx 80kDa band observed in fetal Mouse Kidney lysates (calculated MW of 73.2kDa according to Human NP_005060.1) and 72.5kDa according to Mouse NP_035507.2. Recommended concentration: 0.5-2ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of fetal Mouse kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of fetal Mouse kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
Uniprot Description
SIM2: Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 21q22.13
Cellular Component: nucleoplasm
Molecular Function: DNA binding; protein heterodimerization activity; transcription factor activity
Biological Process: cell differentiation; embryonic pattern specification; lung development; negative regulation of transcription from RNA polymerase II promoter; nervous system development; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
Research Articles on SIM2
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Product Notes
The SIM2 sim2 (Catalog #AAA422567) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SIM2 Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIM2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 80kDa band observed in fetal Mouse Kidney lysates (calculated MW of 73.2kDa according to Human NP_005060.1) and 72.5kDa according to Mouse NP_035507.2. Recommended concentration: 0.5-2ug/ml. Researchers should empirically determine the suitability of the SIM2 sim2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SDLLYTPSYS. It is sometimes possible for the material contained within the vial of "SIM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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