Western Blot (WB)
((2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SPHK1 Polyclonal Antibody | anti-SPHK1 antibody
Goat anti-SPHK1 Antibody
Western Blot: approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, lipid; EC 2.7.1.91; Lipid Metabolism - sphingolipid
Chromosomal Location of Human Ortholog: 17q25.2
Cellular Component: axon; cytoplasm; cytosol; nucleus; plasma membrane; synaptic vesicle
Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; magnesium ion binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity
Biological Process: activation of NF-kappaB transcription factor; blood vessel development; brain development; calcium-mediated signaling; cellular response to starvation; cyclooxygenase pathway; female pregnancy; inflammatory response; lipid phosphorylation; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; positive regulation of NF-kappaB import into nucleus; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; protein folding; regulation of interleukin-1 beta production; response to amine stimulus; response to ATP; response to magnesium ion; response to progesterone stimulus; signal transduction; sphingoid catabolic process; sphingolipid biosynthetic process; sphingosine biosynthetic process; sphingosine metabolic process
Research Articles on SPHK1
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Product Notes
The SPHK1 sphk1 (Catalog #AAA421620) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SPHK1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SPHK1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3ug/ml. Researchers should empirically determine the suitability of the SPHK1 sphk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DVDLESEKYR RLGE. It is sometimes possible for the material contained within the vial of "SPHK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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