Western Blot (WB)
(All lanes : Anti-H3f3b Antibody (C-Term) at 1:2000 dilutionLane 1: mouse lung lysateLane 2: mouse cerebellum lysateLane 3: mouse spleen lysateLane 4: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit H3f3b Polyclonal Antibody | anti-H3f3b antibody
H3f3b Antibody (C-Term)
Reactivity
MousePredicted: Human, Bovine, Rat, Chicken, Zebrafish, Xenopus, C.Elegans, Drosophila, Pig, Rabbit
Applications
Western Blot, ELISA
Synonyms
H3f3b; Polyclonal Antibody; H3f3b Antibody (C-Term); Histone H33; H3f3a; H33a; anti-H3f3b antibody
Host
Rabbit
Reactivity
Mouse
Predicted: Human, Bovine, Rat, Chicken, Zebrafish, Xenopus, C.Elegans, Drosophila, Pig, Rabbit
Predicted: Human, Bovine, Rat, Chicken, Zebrafish, Xenopus, C.Elegans, Drosophila, Pig, Rabbit
Clonality
Polyclonal
Isotype
Ig
Specificity
This H3f3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-136 amino acids from mouse H3f3b.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
136
Applicable Applications for anti-H3f3b antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Source
Human
Antigen Region
103-136
Antigen Type
Synthesized Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-H3f3b Antibody (C-Term) at 1:2000 dilutionLane 1: mouse lung lysateLane 2: mouse cerebellum lysateLane 3: mouse spleen lysateLane 4: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-H3f3b Antibody (C-Term) at 1:2000 dilutionLane 1: mouse lung lysateLane 2: mouse cerebellum lysateLane 3: mouse spleen lysateLane 4: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-H3f3b antibody
Description: Purified Rabbit Polyclonal Antibody (Pab)
Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
References
Hraba-Renevey S.,et al.Nucleic Acids Res. 17:2449-2461(1989). Bramlage B.,et al.Differentiation 62:13-20(1997). Lopez-Alanon D.M.,et al.DNA Cell Biol. 16:639-644(1997). Carninci P.,et al.Science 309:1559-1563(2005). Mancini P.,et al.J. Mol. Evol. 59:458-463(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15328
NCBI Official Full Name
histone H3.3
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3f3a
UniProt Synonym Gene Names
H3.3a
H3f3b; H3.3b
H3f3b; H3.3b
UniProt Entry Name
H33_MOUSE
Similar Products
Product Notes
The H3f3b h3f3a (Catalog #AAA9232188) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The H3f3b Antibody (C-Term) reacts with Mouse Predicted: Human, Bovine, Rat, Chicken, Zebrafish, Xenopus, C.Elegans, Drosophila, Pig, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's H3f3b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the H3f3b h3f3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H3f3b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
