Goat NOS1 Polyclonal Antibody | anti-NOS1 antibody

Goat anti-NOS1 Antibody

Cat. Num. AAA420845

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Tested: Human, Marmoset, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: NOS1; Polyclonal Antibody; Goat anti-NOS1 Antibody; nitric oxide synthase 1 (neuronal); NOS; neuronal nitric oxide synthase; PnNOS; penile neuronal nitric oxide synthase; penile neuronal NOS; IHPS1; nNOS; nitric oxide synthase 1; neuronal; NOS1 antibody; nitric oxide synthase 1 (neuronal) antibody; NOS antibody; neuronal nitric oxide synthase antibody; PnNOS antibody; penile neuronal nitric oxide synthase antibody; penile neuronal NOS antibody; IHPS1 antibody; nNOS antibody; neuronal antibody; anti-NOS1 antibody

• Host: Goat
• Reactivity: Tested: Human, Marmoset, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ESKKDTDEVFSS
• Sequence Length: 1434

• Applicable Applications for anti-NOS1 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: This antibody has been successfully used in IHC for mouse olfactory bulb as described in the following paper: Fried et al, Cell Tissue Res.2010 Mar;339(3): 463-79; PMID: 20140458. Successful results were also reported by anonymous customers in Rat Cerebral Cortex and in Marmoset Cerebellum.; Western Blot: Approx 160-170kDa band observed in Human Skeletal Muscle and Mouse Brain lysates (calculated MW of 161kDa according to NP_000611.1). Recommended concentration: 0.1-0.3ug/ml.; Immunofluorescence: This product has been successfully used for IF as reported (PMID: 20140458).
• Immunogen: Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• Molecular Weight: 164,779 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS
• UniProt Entry Name: NOS1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: azurophil granule; caveola; cytoplasm; cytoskeleton; cytosol; dendritic spine; lipid raft; mitochondrial outer membrane; mitochondrion; nuclear membrane; perinuclear region of cytoplasm; photoreceptor inner segment; postsynaptic density; protein complex; sarcolemma; sarcoplasmic reticulum; synapse; T-tubule; vesicle membrane; Z disc

Molecular Function: cadmium ion binding; calmodulin binding; enzyme binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; sodium channel regulator activity

Biological Process: aging; arginine catabolic process; behavioral response to cocaine; cell redox homeostasis; exogenous drug catabolic process; female pregnancy; multicellular organismal response to stress; myoblast fusion; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of heart contraction; negative regulation of hydrolase activity; negative regulation of insulin secretion; negative regulation of potassium ion transport; negative regulation of serotonin uptake; negative regulation of vasoconstriction; neurotransmitter biosynthetic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; reduction of cytosolic calcium ion concentration; regulation of neurogenesis; regulation of sensory perception of pain; regulation of sodium ion transport; response to activity; response to estrogen stimulus; response to ethanol; response to heat; response to hypoxia; response to lead ion; response to lipopolysaccharide; response to nicotine; response to peptide hormone stimulus; response to vitamin E; striated muscle contraction

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1