Rabbit PIWL1 Polyclonal Antibody | anti-PIWL1 antibody

PIWL1 Antibody

Cat. Num. AAA9611269

• Gene Names: PIWIL1; HIWI; MIWI; PIWI; CT80.1
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PIWL1; Polyclonal Antibody; PIWL1 Antibody; HIWI; MIWI; Piwi (Drosophila) like 1; PIWI; Piwi homolog; Piwi like 1 (drosophila); Piwi like 1; Piwi-like protein 1; PIWIL1; PIWL1_HUMAN; anti-PIWL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIWL1 Antibody detects endogenous levels of PIWL1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 829

• Applicable Applications for anti-PIWL1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human PIWL1.
• Tissue Specificity: Expressed in spermatocytes and spermatids. Also detected in prostate cancer (at protein level). Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3: Ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse testis tissue, using PIWL1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-PIWL1 antibody

Description: Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1.

Post Translational Modifications: Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in late spermatids, leading to its degradation (PubMed:28552346). Ubiquitination only takes place following piRNA-binding in adult testis (By similarity). Ubiquitination and degradation in late spermatogenesis by APC/C is probably required to release RNF8 from the cytoplasm and promote histone to protamine exchange by RNF8 (By similarity).

Subunit Structure: Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes; this interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Interacts with CLOCK. Interacts with MOV10L1. Interacts with ANAPC10; interaction oly takes place following piRNA-binding. Interacts with RNF8; leading to sequester RNF8 in the cytoplasm. Interacts with TEX19 (By similarity).

Similarity: The PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs (PubMed:21193640, PubMed:21465557). The MID region is required for recognition of uridine in the first position of piRNAs (g1U preference, also named 1U-bias) (By similarity). The D-box (destruction box) acts as a recognition signal for association with the APC/C complex, ubiquitination and degradation. Belongs to the argonaute family. Piwi subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 300244501
• NCBI GeneID: 9271
• NCBI Accession #: NP_001177900.1
• NCBI GenBank Nucleotide #: NP_001177900.1
• UniProt Accession #: Q96J94
• Molecular Weight: Observed: 90 kDa; Predicted: 99 kDa
• NCBI Official Full Name: piwi-like protein 1 isoform 2
• NCBI Official Synonym Full Names: piwi like RNA-mediated gene silencing 1
• NCBI Official Symbol: PIWIL1
• NCBI Official Synonym Symbols: HIWI; MIWI; PIWI; CT80.1
• NCBI Protein Information: piwi-like protein 1
• UniProt Protein Name: Piwi-like protein 1
• UniProt Gene Name: PIWIL1
• UniProt Synonym Gene Names: HIWI
• UniProt Entry Name: PIWL1_HUMAN

NCBI Description

This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]