Immunohistochemistry (IHC)
(Anti-TRIM25 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Rabbit anti-Human, Mouse TRIM25 Polyclonal Antibody | anti-TRIM25 antibody
Rabbit Polyclonal (IgG) to Human TRIM25
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Immunohistochemistry (IHC)
(Anti-TRIM25 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-TRIM25 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence of TRIM25 in HeLa cells with TRIM25 antibody at 20 ug/ml.)
Immunocytochemistry (ICC)
(Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/ml.)
Immunocytochemistry (ICC)
(Immunocytochemistry of TRIM25 in HeLa cells with TRIM25 antibody at 20 µg/ml.)
Western Blot (WB)
(Western blot of TRIM25 in HeLa cell lysate in (A) the absence and (B) absence of blocking peptide with TRIM25 antibody at 1 ug/ml.)
Western Blot (WB)
(Western blot of TRIM25 in HeLa cell lysate in (A) the absence and (B) absence of blocking peptide with TRIM25 antibody at 1 ug/ml.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.
Protein type: Ubiquitin conjugating system; EC 6.3.2.n3; Nuclear receptor co-regulator; Ubiquitin ligase; EC 6.3.2.19; Ligase; Transcription factor
Chromosomal Location of Human Ortholog: 17q23.2
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; defense response to virus; negative regulation of interferon type I production
Research Articles on TRIM25
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Product Notes
The TRIM25 trim25 (Catalog #AAA242165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human TRIM25 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM25 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA). ELISA, ICC (20 ug/ml), IHC-P (10 ug/ml), WB (1 - 2 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the TRIM25 trim25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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