Goat CLPP Polyclonal Antibody | anti-CLPP antibody

Goat anti-CLPP Antibody

Cat. Num. AAA420213

• Gene Names: CLPP; DFNB81; PRLTS3
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CLPP; Polyclonal Antibody; Goat anti-CLPP Antibody; ClpP caseinolytic protease; ATP-dependent; proteolytic subunit homolog (E. coli); ATP-dependent proteolytic subunit E. coli homolog; ATP-dependent protease ClpAP (E. coli); proteolytic subunit; human; ClpP (caseinolytic protease; prote; CLPP antibody; proteolytic subunit homolog (E. coli) antibody; ATP-dependent proteolytic subunit E. coli homolog antibody; human antibody; E. coli) homolog antibody; ClpP caseinolytic peptidase; ATP-dependent protease ClpAP; proteolytic subunit homolog antibody; caseinolytic peptidase; proteolytic subunit antibody; anti-CLPP antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: EAAPAAEPVPAST
• Sequence Length: 277

• Applicable Applications for anti-CLPP antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 1000.; Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes. Recommended concentration, 2-4ug/ml.; Western Blot: Approx 26kDa band observed in Human Heart (calculated MW of 30.2kDa according to NP_006003.1). Recommended for use at 1-3ug/ml.
• Immunogen: Peptide with sequence C-EAAPAAEPVPAST, from the C Terminus of the protein sequence according to NP_006003.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 5174419
• NCBI GeneID: 8192
• NCBI Accession #: NP_006003.1
• NCBI GenBank Nucleotide #: NM_006012.2
• Molecular Weight: 30,180 Da
• NCBI Official Full Name: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• NCBI Official Synonym Full Names: caseinolytic mitochondrial matrix peptidase proteolytic subunit
• NCBI Official Symbol: CLPP
• NCBI Official Synonym Symbols: DFNB81; PRLTS3
• NCBI Protein Information: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• UniProt Protein Name: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• Protein Family: ATP-dependent Clp protease proteolytic
• UniProt Gene Name: CLPP
• UniProt Entry Name: CLPP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]

Uniprot Description

CLPP: Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Belongs to the peptidase S14 family.

Protein type: Protease; Mitochondrial; EC 3.4.21.92

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: identical protein binding; peptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: protein homooligomerization; proteolysis involved in cellular protein catabolic process

Disease: Perrault Syndrome 3