Human Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial ELISA Kit | CLPP elisa kit

Human Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial ELISA Kit

Cat. Num. AAA289701

• Gene Names: CLPP; DFNB81; PRLTS3
• Reactivity: Human
• Synonyms: Putative ATP-dependent Clp protease proteolytic subunit; mitochondrial; Human Putative ATP-dependent Clp protease proteolytic subunit; mitochondrial ELISA Kit; Endopeptidase Clp; CLPP; 3.4.21.92; CLPP elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human ATP-dependent Clp protease proteolytic subunit, mitochondrial
• Sequence Length: 277

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 1.56 ng/mL - 100 ng/mL
• Sensitivity: < 0.54 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.9%
• Inter-assay Precision: Inter-Assay CV: <=8.2%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLPP elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5174419
• NCBI GeneID: 8192
• NCBI Accession #: NP_006003.1
• NCBI GenBank Nucleotide #: NM_006012.2
• UniProt Accession #: Q16740; B2R4W5
• Molecular Weight: 30,180 Da
• NCBI Official Full Name: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• NCBI Official Synonym Full Names: caseinolytic mitochondrial matrix peptidase proteolytic subunit
• NCBI Official Symbol: CLPP
• NCBI Official Synonym Symbols: DFNB81; PRLTS3
• NCBI Protein Information: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• UniProt Protein Name: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• Protein Family: Putative ATP-dependent Clp protease proteolytic
• UniProt Gene Name: CLPP
• UniProt Entry Name: CLPP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]

Uniprot Description

CLPP: Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Belongs to the peptidase S14 family.

Protein type: EC 3.4.21.92; Mitochondrial; Protease

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: identical protein binding; peptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: protein homooligomerization; proteolysis involved in cellular protein catabolic process

Disease: Perrault Syndrome 3