Monkey Albumin ELISA Kit | ALB elisa kit

Monkey Albumin ELISA Kit

Cat. Num. AAA742791

• Gene Names: ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
• Reactivity: Monkey
• Synonyms: Albumin; Monkey Albumin ELISA Kit; ALB elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5-1000ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALB elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This ALB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey ALB. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY ALB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALB. Standards or samples are then added to the microtiter plate wells and ALB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALB are added to each well to “sandwich” the ALB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALB concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37222202
• NCBI GeneID: 213
• Molecular Weight: 47,360 Da
• NCBI Official Full Name: ALB, partial
• NCBI Official Synonym Full Names: albumin
• NCBI Official Symbol: ALB
• NCBI Official Synonym Symbols: FDAH; ANALBA; PRO0883; PRO0903; PRO1341
• NCBI Protein Information: serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42
• UniProt Protein Name: Serum albumin
• Protein Family: Albumin
• UniProt Gene Name: ALB
• UniProt Synonym Gene Names: GIG20; GIG42; PRO0903; PRO1708; PRO2044; PRO2619; PRO2675; UNQ696/PRO1341
• UniProt Entry Name: ALBU_HUMAN

NCBI Description

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Carrier

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus

Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding

Biological Process: receptor-mediated endocytosis; platelet activation; sodium-independent organic anion transport; bile acid metabolic process; maintenance of mitochondrion localization; lipoprotein metabolic process; hemolysis by symbiont of host red blood cells; cellular response to starvation; response to mercury ion; bile acid and bile salt transport; response to organic substance; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; transmembrane transport; positive regulation of circadian sleep/wake cycle, non-REM sleep; response to nutrient; negative regulation of apoptosis

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic