MLK1 active protein

Recombinant Human MLK1 (N-GST tag)

Cat. Num. AAA4160226

• Gene Names: MAP3K9; MLK1; MEKK9; PRKE1
• Purity: 70%-90%
• Synonyms: MLK1; Recombinant Human MLK1 (N-GST tag); MAP3K9; MEKK9; PRKE1; MLK1 active protein

• Host: Insect Cells
• Purity/Purification: 70%-90%
• Form/Format: Liquid; 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 11553

• Bioactivity: The specific activity of MLK1 was determined to be 116 nmol/min/mg as per activity assay protocol.
• Preparation and Storage: Store product at-70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Related Product Information for MLK1 active protein

MLK1 or Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH-terminal kinase (JNK) pathway. The catalytic domain of MLK1 has amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition, MLK1 contain 2 leu/ile-zipper motifs and a basic sequence near its C-termini. MLK1 is threonine (and possibly serine) phosphorylated at multiple sites in the activation loop, with phosphorylation of Thr312 required for full activation. 

NCBI and Uniprot Product Information

• NCBI GI #: 1677501349
• NCBI GeneID: 4293
• NCBI Accession #: NM_033141.4
• NCBI GenBank Nucleotide #: NM_033141
• UniProt Accession #: P80192
• Molecular Weight: 77.0 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase kinase kinase 9 (MAP3K9), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 9
• NCBI Official Symbol: MAP3K9
• NCBI Official Synonym Symbols: MLK1; MEKK9; PRKE1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 9
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 9
• UniProt Gene Name: MAP3K9
• UniProt Synonym Gene Names: MLK1; PRKE1
• UniProt Entry Name: M3K9_HUMAN

Uniprot Description

MLK1: a TKL kinase of the MLK family. A dual leucine zipper kinase. Expressed in neuronal cells and may act between Rac1/Cdc42 and MKK4 and -7 in death signaling.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 14q24.2

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; JUN kinase kinase kinase activity; protein-tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; activation of JNK activity; activation of JNKK activity