Testing Data
Ly-6A/E (Sca-1) Secondary Antibody
Rat Anti-Mouse Ly-6A/E-FITC
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Ly-6A/E (Sca-1); Monoclonal Secondary Antibody; Rat Anti-Mouse Ly-6A/E-FITC; Rat Anti-Mouse Ly-6A/E - FITC; Rat Anti-Mouse Ly-6A/E (Sca-1); Ly-6A/E (Sca-1) secondary antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
D7
Specificity
Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18 kDa
Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.^1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.^4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E^+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E^+ lymphocytes.^1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.^1, 8, 9 Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses,^9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.^3, 13
Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.^1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.^4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E^+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E^+ lymphocytes.^1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.^1, 8, 9 Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses,^9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.^3, 13
Form/Format
Fluorescein (FITC) Conjugate
Applicable Applications for Ly-6A/E (Sca-1) secondary antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
Application Notes
Research Application(s): In vitro lymphocyte activation
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Immunogen
IL-2-dependent mouse T-cell line CTL-L
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
Testing Data
Testing Data
References
1. Ortega, G., P.E. Korty, E.M. Shevach, and T.R. Malek. 1986. J. Immunol. 137:3240-3246.
2. Palfeee, R.G.E., F.J. Dumont, and U. Hammerling. 1986. Immunogenetics 23:197-207.
3. Rock, K.L., H. Reiser, A. Bamezai, J. McGrew, and B. Benacerraf. 1989. Immunol. Rev. 111:195-224.
4. Moore, T., M. Bennett, and V. Kumar. 1995. J. Immunol. 154:1653-1663.
5. Ito, M., K. Anan, M. Masawa, S. Kai, and H. Hara. 1996. Stem Cells 14:412-418.
6. Yonemura, Y., H. Ku, S.D. Lyman, and M. Ogawa. 1997. Blood 89: 1915-1921.
7. Codias, E.K., and C. Cray, R.D. Baler, R.B. Levy, and T.R. Malek. 1989. Immunogenetics 29:98-107.
8. Malek, T.R., K.M. Danis, and E.K. Codias. 1989. J. Immunol. 142:1929-1936.
9. Codias, E.K., and T.R. Malek. 1990. J. Immunol. 144:2197-2204.
10. Malek, T.R., G. Ortega, C. Chan, R.A. Kroczek, and E.M. Shevach. 1986. J. Exp. Med. 164:709-722.
11. Codias, E.K., J.E. Rutter, T.J. Fleming, and T.R. Malek. 1990. J. Immunol. 145:1407-1414.
12. Ivanov, V., T.J. Fleming, and T.R. Malek. 1994. J. Immunol. 153:2394-2406.
13. Flood, P.M., J.P. Dougherty, and Y. Ron. 1993. J. Exp. Med. 172:115-120.
2. Palfeee, R.G.E., F.J. Dumont, and U. Hammerling. 1986. Immunogenetics 23:197-207.
3. Rock, K.L., H. Reiser, A. Bamezai, J. McGrew, and B. Benacerraf. 1989. Immunol. Rev. 111:195-224.
4. Moore, T., M. Bennett, and V. Kumar. 1995. J. Immunol. 154:1653-1663.
5. Ito, M., K. Anan, M. Masawa, S. Kai, and H. Hara. 1996. Stem Cells 14:412-418.
6. Yonemura, Y., H. Ku, S.D. Lyman, and M. Ogawa. 1997. Blood 89: 1915-1921.
7. Codias, E.K., and C. Cray, R.D. Baler, R.B. Levy, and T.R. Malek. 1989. Immunogenetics 29:98-107.
8. Malek, T.R., K.M. Danis, and E.K. Codias. 1989. J. Immunol. 142:1929-1936.
9. Codias, E.K., and T.R. Malek. 1990. J. Immunol. 144:2197-2204.
10. Malek, T.R., G. Ortega, C. Chan, R.A. Kroczek, and E.M. Shevach. 1986. J. Exp. Med. 164:709-722.
11. Codias, E.K., J.E. Rutter, T.J. Fleming, and T.R. Malek. 1990. J. Immunol. 145:1407-1414.
12. Ivanov, V., T.J. Fleming, and T.R. Malek. 1994. J. Immunol. 153:2394-2406.
13. Flood, P.M., J.P. Dougherty, and Y. Ron. 1993. J. Exp. Med. 172:115-120.
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Product Notes
The Ly-6A/E (Sca-1) (Catalog #AAA673478) is a Secondary Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse Ly-6A/E-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ly-6A/E (Sca-1) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot. Research Application(s): In vitro lymphocyte activation Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the Ly-6A/E (Sca-1) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly-6A/E (Sca-1), Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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