Rat anti-Mouse, Rat p19Arf Monoclonal Antibody | anti-p19Arf antibody

Anti-Mouse p19Arf (Exon 2) Antibody

Cat. Num. AAA4156721

• Reactivity: Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: p19Arf; Monoclonal Antibody; Anti-Mouse p19Arf (Exon 2) Antibody; Mouse p19Arf; anti-p19Arf antibody

• Host: Rat
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This is a Protein G purified antibody directed against mouse p19Arf protein. No reactivity is observed against human or hamster homologues. The epitope was putatively mapped to amino acids 62-75 of mouse p19Arf. BLAST analysis indicates that no significant sequence homology exists for this sequence with p19Arf homologues from other sources. The homologue from rat only shows 11 of 14 identities (78% homology). No additional information is available for reactivity with p19Arf protein from other sources.
• Purity/Purification: Protein G Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 155

• Applicable Applications for anti-p19Arf antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: This antibody has been tested for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band approximately 19 kDa in size corresponding to mouse p19Arf by western blotting in the appropriate cell line or lysate.
• Immunogen: This protein G purified monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 54-75 of mouse p19Arf protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-p19Arf antibody

The Ink4a-Arf locus encodes two tumor suppressor proteins, p16Ink4a and p19Arf. Expression of Ink4a and Arf is regulated by distinct promoters upstream of alternative first exons whose products_recover are spliced to a common second exon translated in alternative reading frames (from which Arf gets its name). The two genes are induced by different stress signals and can be separately mutated or silenced in tumor cells. Targeted disruption of either or both genes in mouse leads to spontaneous tumor formation, which is accelerated by exposure of the mutant animals to chemical carcinogens or ionizing radiation. Inactivation of ARF by mutation, deletion, or epigenetic silencing is observed in many human cancers, underscoring the role of this protein as a potent and ubiquitous tumor suppressor. p19Arf is activated by Myc or mutant Ras. Once activated, p19Arf binds to the p53 negative regulator Mdm2, leading to p53 stabilization and unleashing a p53-dependent transcriptional program that triggers either cell cycle arrest or apoptosis. p19Arf also has p53-independent inhibitory effects on cell proliferation. p19Arf is a nucleolar protein and is implicated in ribosomal biogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 7637746
• NCBI Official Full Name: p19ARF