Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of p14 ARF/p19 ARF (Q99) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)
Rabbit p14 ARF/p19 ARF Polyclonal Antibody | anti-ARF antibody
p14 ARF/p19 ARF (Q99) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
p14 ARF/p19 ARF; Polyclonal Antibody; p14 ARF/p19 ARF (Q99) Polyclonal Antibody; Cyclin-dependent kinase inhibitor 2A; isoform 4; p14ARF; p19ARF; CDKN2A; CDKN2; MLM; anti-ARF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
p14 ARF/p19 ARF (Q99) polyclonal antibody detects endogenous levels of p14 ARF(human)/p19 ARF(Mouse) protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-ARF antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:50-1:200
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 65-121 of Human p19 ARF.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of p14 ARF/p19 ARF (Q99) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of p14 ARF/p19 ARF (Q99) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)
Related Product Information for anti-ARF antibody
The INK4a/ARF locus encodes two unrelated tumor suppressor proteins, p16INK4a and p19ARF that restrain cell growth by modifying the functions of the retinoblastoma protein and p53, respectively. It is among the most frequently mutated tumor suppressor loci in human cancer. Both p16INK4a and p19ARF act as cell proliferation inhibitors. The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53 dependent pathways. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. Expression of p19ARF but not p16INK4a induces apoptosis in Ab-MLV-transformed pre-B cells.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~ 18kDa
Protein Family
Similar Products
Product Notes
The ARF (Catalog #AAA3000764) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p14 ARF/p19 ARF (Q99) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p14 ARF/p19 ARF can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the ARF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p14 ARF/p19 ARF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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