Rabbit anti-Human RAD54 Polyclonal Antibody | anti-RAD54 antibody

Anti-Human RAD54 Antibody

Cat. Num. AAA4157780

• Gene Names: RAD54L; HR54; hHR54; RAD54A; hRAD54
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: RAD54; Polyclonal Antibody; Anti-Human RAD54 Antibody; Human RAD 54; anti-RAD54 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. BLAST analysis indicates 100% homology of the immunizing sequence with RAD54 from human. Cross reactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 747

• Applicable Applications for anti-RAD54 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 1-17 of Human RAD54 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-RAD54 antibody

RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination.

NCBI and Uniprot Product Information

• NCBI GI #: 1495483
• NCBI GeneID: 8438
• NCBI Official Full Name: RAD54
• NCBI Official Synonym Full Names: RAD54 like
• NCBI Official Symbol: RAD54L
• NCBI Official Synonym Symbols: HR54; hHR54; RAD54A; hRAD54
• NCBI Protein Information: DNA repair and recombination protein RAD54-like
• Protein Family: DNA repair and recombination protein

NCBI Description

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008]