Rabbit EAPP Polyclonal Antibody | anti-EAPP antibody

Anti-EAPP Antibody

Cat. Num. AAA415438

• Gene Names: EAPP; BM036; C14orf11
• Reactivity: Human. Predicted: Mouse, Rat.
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: EAPP; Polyclonal Antibody; Anti-EAPP Antibody; anti-EAPP antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat.
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 285

• Applicable Applications for anti-EAPP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:1,000-1:2,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal EAPP (1) antibody was raised against a recombinate human EAPP protein 7-192 aa (BC001245).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-EAPP antibody

This gene encodes a phosphoprotein that interacts with several members of the E2F family of proteins. The protein localizes to the nucleus, and is present throughout the cell cycle except during mitosis. It functions to modulate E2F-regulated transcription and stimulate proliferation. [provided by RefSeq, Jul 2008]

References

(1) Andorfer,P. and Rotheneder,H. EAPP: gatekeeper at the crossroad of apoptosis and p21-mediated cell-cycle arrest. Oncogene 30 (23), 2679-2690 (2011).

NCBI and Uniprot Product Information

• NCBI GI #: 117938268
• NCBI GeneID: 55837
• NCBI Accession #: NP_060923.2
• NCBI GenBank Nucleotide #: NM_018453.3
• Molecular Weight: 32,762 Da
• NCBI Official Full Name: E2F-associated phosphoprotein
• NCBI Official Synonym Full Names: E2F-associated phosphoprotein
• NCBI Official Symbol: EAPP
• NCBI Official Synonym Symbols: BM036; C14orf11
• NCBI Protein Information: E2F-associated phosphoprotein
• UniProt Protein Name: E2F-associated phosphoprotein
• Protein Family: E2F-associated phosphoprotein
• UniProt Gene Name: EAPP
• UniProt Entry Name: EAPP_HUMAN

NCBI Description

This gene encodes a phosphoprotein that interacts with several members of the E2F family of proteins. The protein localizes to the nucleus, and is present throughout the cell cycle except during mitosis. It functions to modulate E2F-regulated transcription and stimulate proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

EAPP: May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression. Interacts with E2F1. The C-terminal half binds the N- terminal of E2F1. Also interacts with E2F2 and E2F3, but not E2F4. Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts.

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleus

Biological Process: positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of cell proliferation