Rabbit anti-Human EAPP Polyclonal Antibody | anti-EAPP antibody

EAPP, CT (EAPP, C14orf11, E2F-associated phosphoprotein) (Biotin)

Cat. Num. AAA6294150

• Gene Names: EAPP; BM036; C14orf11
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EAPP; Polyclonal Antibody; CT (EAPP; C14orf11; E2F-associated phosphoprotein) (Biotin); E2F-associated phosphoprotein; anti-EAPP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EAPP antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EAPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-233 amino acids from the C-terminal region of human EAPP.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EAPP Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the EAPP antibody detected the EAPP protein (arrow).)

Immunohistochemistry (IHC)

(EAPP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EAPP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-EAPP antibody

This gene encodes a phosphoprotein that interacts with several members of the E2F family of proteins. The protein localizes to the nucleus, and is present throughout the cell cycle except during mitosis. It functions to modulate E2F-regulated transcription and stimulate proliferation.

Product Categories/Family for anti-EAPP antibody

Antibodies; KIAA; orf

References

Schwarzmayr, L., et al. Int. J. Biochem. Cell Biol. 40(12):2845-2853(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Lim, J., et al. Cell 125(4):801-814(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 117938268
• NCBI GeneID: 55837
• NCBI Accession #: NP_060923.2
• NCBI GenBank Nucleotide #: NM_018453.3
• UniProt Accession #: Q56P03; Q9BVF4; Q9NWV5; Q9NZ86
• Molecular Weight: 32,762 Da
• NCBI Official Full Name: E2F-associated phosphoprotein
• NCBI Official Synonym Full Names: E2F-associated phosphoprotein
• NCBI Official Symbol: EAPP
• NCBI Official Synonym Symbols: BM036; C14orf11
• NCBI Protein Information: E2F-associated phosphoprotein
• UniProt Protein Name: E2F-associated phosphoprotein
• Protein Family: E2F-associated phosphoprotein
• UniProt Gene Name: EAPP
• UniProt Synonym Gene Names: C14orf11; EAPP
• UniProt Entry Name: EAPP_HUMAN

NCBI Description

This gene encodes a phosphoprotein that interacts with several members of the E2F family of proteins. The protein localizes to the nucleus, and is present throughout the cell cycle except during mitosis. It functions to modulate E2F-regulated transcription and stimulate proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

EAPP: May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression. Interacts with E2F1. The C-terminal half binds the N- terminal of E2F1. Also interacts with E2F2 and E2F3, but not E2F4. Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts.

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleus

Biological Process: positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of cell proliferation