Mouse anti-other His Monoclonal Antibody | anti-His antibody

Anti-His Antibody

Cat. Num. AAA415105

• Reactivity: other
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: IgG fraction
• Synonyms: His; Monoclonal Antibody; Anti-His Antibody; anti-His antibody

• Host: Mouse
• Reactivity: other
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: HIS.H8
• Specificity: His-tag fusion protein
• Purity/Purification: IgG fraction
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 100ug/100ul (varies by lot)

• Applicable Applications for anti-His antibody: Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users.
• Cellular Localization: Depends upon the localization of the parent protein tagged with hexahistidine.
• Tag Information: Tag
• Immunogen: 6x His synthetic peptide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot

Immunofluorescence

Related Product Information for anti-His antibody

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

References

Paluzzi, JP et al. "The heterodimeric glycoprotein hormone, GPA2, GPB5, regulates ion transport across the hindgut of the adult mosquito, Aedes aegypti." PLoS ONE 9:e86386 (2014). PubMed: 24466069. Application: Western Blot.
Tsai, SL et al. "Functional display of complex cellulosomes on the yeast surface via adaptive assembly." ACS Synth Biol 2:14-21 (2013). PubMed: 23656322.
Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate." Dev Biol 365 (2):353-75 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.
Lim, KH et al. "Biotin-assisted folding of streptavidin on the yeast surface." Biotechnol prog 28 (1):276-283 (2012). DOI: 10.1002/btpr.721. PubMed: 21990063. Application: Immunoprecipitation.
Bae, HJ et al. "DBC1 does not function as a negative regulator of SIRT1 in liver cancer." Oncol Lett 4 (5):873-877 (2012). PubMed: 23162614. Application: Western Blot.
Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI1 in regulation of cellular resistance of BCRABL+ chronic myeloid leukemia cells to tyrosine." Proteomics 12 (13:2094-2106 (2012). DOI: 10.1002/pmic.201100553. Application: Western Blot.
Park, UH et al. "Piperine, a Component of Black Pepper, Inhibits Adipogenesis by Antagonizing PPARgamma Activity in 3T3-L1 Cells." J Agric Food Chem 60 (15):3853-3860 (2012). DOI: 10.1021/jf204514a. PubMed: 22463744. Application: Western Blot.
Olmstead, AD et al. "Human subtilase SKI-1, S1P is a master regulator of the HCV Lifecycle and a potential host cell target for developing indirect-acting antiviral agents." PLoS Pathog. 8:e1002468 (2012). PubMed: 22241994.
Mani, T et al. "FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function." Mol Cell Biol 31 (10):183-1996 (2011). DOI: 10.1128/MCB.00609-10. Application: Immunoblot.
Xue, Y et al. "FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair." Hum. Mol. Genet. 17:1641-52 (2008). PubMed: 18285517.
Metzler, M et al. "NMDA receptor function and NMDA receptor-dependent phosphorylation of huntingtin is altered by the endocytic protein HIP1." J Neurosci 27:2298-308 (2007). PubMed: 17329427.