Testing Data
Rabbit anti-Tag Antibody His-Tag Polyclonal Antibody | anti-His-Tag antibody
Rabbit anti His Tag Polyclonal antibody
Reactivity
Tag Antibody
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
The Rabbit IgG is purified by Epitope-Affinity Chromatography
Synonyms
His-Tag; Polyclonal Antibody; Rabbit anti His Tag Polyclonal antibody; Rabbit anti-His tag; HHHHHH; anti-His-Tag antibody
Host
Rabbit
Reactivity
Tag Antibody
Clonality
Polyclonal
Specificity
This antibody recognizes His tagged fusion proteins. It is a tag antibody.
Purity/Purification
The Rabbit IgG is purified by Epitope-Affinity Chromatography
Form/Format
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-His-Tag antibody
ELISA, Western Blot, Immunoprecipitation
Application Notes
Western Blot: 0.5- 2ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Antigen Preparation
A synthetic peptide of HHHHHH conjugated to a carrier protein
Positive Control
His tagged fused proteins
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-His-Tag antibody
Many oligo peptides or tag proteins are widely used as a tag to monitor the protein expression, such as DYKDDDDK, c-Myc, E-tag, His-tag, GFP, GST, and etc. These tag antibodies provide tools to localize gene products in a variety of cell types to study the topology of proteins, and protein complexes, and to identify associated proteins. In addition, this antibody allows characterizing of newly identified, lowing abundance or poorly immunogenic proteins when protein specific antibodies are not available. The His tag antibody is generated according to the 6 amino acid epitope tag (HHHHHH), and it can be used to detect the His tagged fusion proteins.
References
Els Conrath, K et al (2001), J. Biol. Chem. 276: 7346-7350.
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Product Notes
The His-Tag (Catalog #AAA462204) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti His Tag Polyclonal antibody reacts with Tag Antibody and may cross-react with other species as described in the data sheet. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunoprecipitation. Western Blot: 0.5- 2ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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