interleukin 1 receptor antagonist Active Protein | IL-1Ra active protein

Mouse interleukin 1 receptor antagonist

Cat. Num. AAA400078

• Gene Names: IL1R1; P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha
• Purity: Greater than 98.0% as determined by:; (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (b) Gel filtration chromatography under non-denaturing conditions.
• Synonyms: interleukin 1 receptor antagonist; Mouse interleukin 1 receptor antagonist; Mouse Interleukin-1 receptor antagonist (IL-1Ra); IL-1Ra active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.; (b) Gel filtration chromatography under non-denaturing conditions.
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Cys-Arg-Pro-Ser-Gly.
• Sequence Length: 569

• Solubility: It is recommended to reconstitute the lyophilized IL-1Ra in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions containing carrier protein such as BSA, HSA or similar.
• Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the DNAman computer analysis program of protein sequences.
• Dimers and Aggregates: Less than 2% as determined by gel filtration under non-denaturing conditions.
• Endotoxin: Less than 0.05 ng/ug (0.5 EU/ug) of recombinant IL-1a.
• Formulation: Interleukin-1Ra is lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.
• Biological Activity: murine IL-1Ra is capable of inhibitinf IL-1-alpha activity in helper T cell line D10.G4.1
• Preparation and Storage: Lyophilized IL-1Ra although stable at room temperature for 3 weeks, should be stored desiccated below -180 degree C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Related Product Information for IL-1Ra active protein

Interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1a and IL-1b by competitively blocking binding to their type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Recombinant mouse IL-1Ra is a 17.4 kDa protein consisting of 153 amino acid residues. Recombinant monomeric murine IL-1Ra is purified by proprietary chromatographic techniques.

Product Categories/Family for IL-1Ra active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 307046
• NCBI GeneID: 3554
• UniProt Accession #: P14778; Q587I7
• Molecular Weight: 65,402 Da
• NCBI Official Full Name: interleukin 1 receptor
• NCBI Official Synonym Full Names: interleukin 1 receptor, type I
• NCBI Official Symbol: IL1R1
• NCBI Official Synonym Symbols: P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha
• NCBI Protein Information: interleukin-1 receptor type 1; CD121 antigen-like family member A; IL-1R-1; IL-1RT-1; IL-1RT1; interleukin 1 receptor alpha, type I; interleukin-1 receptor alpha; interleukin-1 receptor type I
• UniProt Protein Name: Interleukin-1 receptor type 1
• UniProt Gene Name: IL1R1
• UniProt Synonym Gene Names: IL1R; IL1RA; IL1RT1; IL-1R-1; IL-1RT-1; IL-1RT1; IL-1R-alpha; mIL-1R1; mIL-1RI; sIL-1R1; sIL-1RI
• UniProt Entry Name: IL1R1_HUMAN

NCBI Description

This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12. [provided by RefSeq, Dec 2013]

Uniprot Description

IL-1RA: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Belongs to the interleukin-1 receptor family.

Protein type: Membrane protein, integral; EC 2.7.11.25; Receptor, cytokine

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: interleukin-1, Type I, activating receptor activity; signal transducer activity; protein binding; transmembrane receptor activity; protease binding; platelet-derived growth factor receptor binding; interleukin-1 receptor activity

Biological Process: cell surface receptor linked signal transduction; regulation of inflammatory response; immune response