Sphingosine Kinase 2, Long Form, Human Peptide | SPHK2 peptide

Sphingosine Kinase 2, Long Form, Human

Cat. Num. AAA396454

• Gene Names: SPHK2; SK 2; SK-2; SPK 2; SPK-2
• Applications: Antibody Function Blocking
• Synonyms: Sphingosine Kinase 2; Long Form; Human; Sphk2-L; N-terminal-extended Sphingosine Kinase 2; SPHK2 peptide

• Host: Rabbit
• Isotype: IgG
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 654

• Applicable Applications for SPHK2 peptide: Antibody Function Blocking
• Comments: Blocking peptide for use with Human Sphingosine Kinase 2, Long Form antibodies.
• Instruction: Incubate antibody neat with at least a 50 fold stoichiometric excess of blocking peptide at 37 Deg. C for 20 minutes (molecular weights of peptide and antibody are ~2.5 kDa and ~160 kDa, respectively). Antibody can then be diluted to a concentration suitable for Western Blot.; ; Example: 10ul or 10ug of Exalpha’s rabbit anti-human Sphingosine Kinase 2 , Long form is added to 10ug of blocking peptide for a total volume of 20 ul. The mixture is allowed to incubate for 20 minutes at 37 Deg. C prior to dilution in suitable buffer (for Western Blot, etc.).
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles; Products are stable for one year from purchase when stored properly.

Related Product Information for SPHK2 peptide

Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.

Product Categories/Family for SPHK2 peptide

Blocking Peptide; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 323462187
• NCBI GeneID: 56848
• NCBI Accession #: NP_001191088.1
• NCBI GenBank Nucleotide #: NM_001204159.2
• UniProt Accession #: Q9NRA0; Q9BRN1; Q9H0Q2; Q9NWU7; B4DU87
• Molecular Weight: 69,217 Da
• NCBI Official Full Name: sphingosine kinase 2 isoform a
• NCBI Official Synonym Full Names: sphingosine kinase 2
• NCBI Official Symbol: SPHK2
• NCBI Official Synonym Symbols: SK 2; SK-2; SPK 2; SPK-2
• NCBI Protein Information: sphingosine kinase 2; sphingosine kinase type 2
• UniProt Protein Name: Sphingosine kinase 2
• Protein Family: Sphingosine kinase
• UniProt Gene Name: SPHK2
• UniProt Synonym Gene Names: SK 2; SPK 2
• UniProt Entry Name: SPHK2_HUMAN

NCBI Description

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. Ref.8

Catalytic activity: ATP + sphinganine = ADP + sphinganine 1-phosphate. Ref.1ATP + sphingosine = ADP + sphingosine 1-phosphate. Ref.1

Cofactor: Magnesium

By similarity.

Enzyme regulation: Inhibited by sulfatide. Ref.8

Subcellular location: Isoform 1: Cytoplasm. Membrane Ref.6 Ref.8. Isoform 2: Lysosome membrane Ref.6 Ref.8.

Sequence similarities: Contains 1 DAGKc domain.

Biophysicochemical propertiesKinetic parameters:KM=3.4 µM for sphingosine Ref.1pH dependence:Optimum pH is 7.5.