Mouse anti-Human, Mouse Rit Monoclonal Antibody | anti-Rit antibody

Rit

Cat. Num. AAA395250

• Gene Names: Rit1; Rit; RIBB; ROC1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Synonyms: Rit; Monoclonal Antibody; anti-Rit antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 14G7
• Form/Format: Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide
• Sequence Length: 219

• Applicable Applications for anti-Rit antibody: Western Blot (WB)
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human Rit protein and mouse myeloma cells.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis)

Related Product Information for anti-Rit antibody

Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Product Categories/Family for anti-Rit antibody

Monoclonal Antibody; G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6677755
• NCBI GeneID: 19769
• NCBI Accession #: NP_033095.1
• NCBI GenBank Nucleotide #: NM_009069.4
• UniProt Accession #: P70426
• Molecular Weight: 35
• NCBI Official Full Name: GTP-binding protein Rit1 isoform 1
• NCBI Official Synonym Full Names: Ras-like without CAAX 1
• NCBI Official Symbol: Rit1
• NCBI Official Synonym Symbols: Rit; RIBB; ROC1
• NCBI Protein Information: GTP-binding protein Rit1; ras-like without CAAX protein 1; RAS-like protein expressed in many tissues
• UniProt Protein Name: GTP-binding protein Rit1
• UniProt Gene Name: Rit1
• UniProt Synonym Gene Names: Rit
• UniProt Entry Name: RIT1_MOUSE

Uniprot Description

Function: Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation

By similarity.

Enzyme regulation: Alternate between an inactive form bound to GDP and an active form bound to GTP

By similarity.

Subunit structure: Interacts with MLLT4, the C-terminal domain of RALGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with RAF1. BARF and RAF1 association is dependent upon the GTP-bound state

By similarity. Interacts with RGL3. Ref.4

Subcellular location: Cell membrane.

Tissue specificity: Expressed in many tissues.

Miscellaneous: Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation

By similarity.Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are very much 10-fold faster than those of most Ras subfamily members

By similarity.

Sequence similarities: Belongs to the small GTPase superfamily. Ras family.