ITK recombinant protein

Recombinant ITK (352-620) protein

Cat. Num. AAA389406

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Synonyms: ITK; Recombinant ITK (352-620) protein; IL-2 Inducible T-Cell Kinase; Tyrosine-Protein Kinase LYK; EMT; LYK; LPFS1; ITK recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant ITK (352-620) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant ITK (352-620) protein that includes amino acids 352-620 of human ITK protein (accession number NP_005537.3) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant ITK (352-620) protein gel 10% SDS-PAGE with Coomassie staining MW: 31.8 kDa Purity: >95%)

Related Product Information for ITK recombinant protein

Background: ITK (IL2 Inducible T Cell Kinase) is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response and regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. The phosphorylation leads to ITK autophosphorylation and full activation. Once activated, it phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. It also phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Diseases associated with ITK include Lymphoproliferative Syndrome 1 and Agammaglobulinemia, X-Linked.

Product Categories/Family for ITK recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 15718680
• NCBI GeneID: 3702
• NCBI Accession #: NP_005537.3
• NCBI GenBank Nucleotide #: NM_005546.3
• UniProt Accession #: Q08881; Q32ML7; B2R752
• Molecular Weight: 71,831 Da
• NCBI Official Full Name: tyrosine-protein kinase ITK/TSK
• NCBI Official Synonym Full Names: IL2 inducible T-cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase
• UniProt Entry Name: ITK_HUMAN

NCBI Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]