PRKAA1 recombinant protein

Recombinant PRKAA1 complex

Cat. Num. AAA389384

• Synonyms: PRKAA1; Recombinant PRKAA1 complex; 5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; PRKAA1 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant PRKAA1 Complex is supplied 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PRKAA1 Complex 10% SDS-PAGE with Coomassie blue staining MW of PRKAA1: 65.3 kDa MW of PRKAB2: 30 kDa MW of PRKAG1: 37.6 kDa Purity: >90%)

Related Product Information for PRKAA1 recombinant protein

Short Description: Recombinant PRKAA1 Complex that includes full-length human PRKAA1 protein (accession number NP_996790.3) with a N-terminal DYKDDDDK tag and full-length human PRKAB2 protein (accession number NP_005390.1) without tag and full-length human PRKAG1 protein (accession number NP_002724.1) without tag was expressed in Sf9 cells. The molecular weights of PRKAA1, PRKAB2, PRKAG1 are 65.3 kDa, 30 kDa and 37.6 kDa, respectively.

Background: PRKAA1, Protein Kinase AMP-Activated Catalytic Subunit Alpha 1, a member of the ser/thr protein kinase family, is a component of the 5'-prime-AMP-activated protein kinase (AMPK)(contain PRKAA1,PRKAB2, PRKAG1), it is an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes, protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively; regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A.

Product Categories/Family for PRKAA1 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• Molecular Weight: Molecular weights of PRKAA1, PRKAB2, PRKAG1 are 65.3 kDa, 30 kDa and 37.6 kDa