SSRP1/FACT recombinant protein

Recombinant SSRP1/FACT p80 protein

Cat. Num. AAA389151

• Gene Names: SSRP1; FACT; T160; FACT80
• Synonyms: SSRP1/FACT; Recombinant SSRP1/FACT p80 protein; T160; FACT80; SSRP1/FACT recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant SSRP1/FACT p80 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant SSRP1 / FACT p80 protein gel 7.5% SDS-PAGE Coomassie staining MW: 82.2 kDa Purity: >95% )

Related Product Information for SSRP1/FACT recombinant protein

Short Description: Recombinant human SSRP1/FACT p80 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_003137.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 82.2 kDa.

Background: SSRP1 (Structure Specific Recognition Protein 1), also known as FACT p80 (Facilitates Chromatin Transcription Complex 80 KDa Subunit), is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of Ser-392 of p53/TP53 via its association with CK2 (casein kinase II).

Product Categories/Family for SSRP1/FACT recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 4507241
• NCBI GeneID: 6749
• NCBI Accession #: NP_003137.1
• NCBI GenBank Nucleotide #: NM_003146.2
• UniProt Accession #: Q08945; Q5BJG8
• Molecular Weight: 81,075 Da
• NCBI Official Full Name: FACT complex subunit SSRP1
• NCBI Official Synonym Full Names: structure specific recognition protein 1
• NCBI Official Symbol: SSRP1
• NCBI Official Synonym Symbols: FACT; T160; FACT80
• NCBI Protein Information: FACT complex subunit SSRP1; hSSRP1; FACTp80; cisplatin-DNA SSRP; FACT 80 kDa subunit; high mobility group box; structure-specific recognition protein 1; facilitates chromatin remodeling 80 kDa subunit; recombination signal sequence recognition protein 1
• UniProt Protein Name: FACT complex subunit SSRP1
• UniProt Gene Name: SSRP1
• UniProt Synonym Gene Names: FACT80; FACT 80 kDa subunit; FACTp80; hSSRP1
• UniProt Entry Name: SSRP1_HUMAN

Uniprot Description

SSRP1: a subunit of the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63.

Protein type: Transcription initiation complex; Transcription, coactivator/corepressor; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: nucleoplasm; cytoplasm; nucleolus; chromosome; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; chromatin remodeling; regulation of transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair; DNA replication