Rabbit CREB Polyclonal Antibody | anti-CREB antibody

CREB Antibody

Cat. Num. AAA840162

• Gene Names: Creb1; Creb
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: CREB; Polyclonal Antibody; CREB Antibody; CREB-1; cAMP-response Element-binding Protein-1; CREB1; MGC9284; anti-CREB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 341

• Applicable Applications for anti-CREB antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 43 kDa CREB from samples of human, mouse and rat origins.
• Immunogen: Synthetic peptide surrounding amino acid 109 of rat CREB
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-CREB antibody

Background: Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.

NCBI and Uniprot Product Information

• NCBI GI #: 13591924
• NCBI GeneID: 81646
• NCBI Accession #: NP_112279.1
• NCBI GenBank Nucleotide #: NM_031017.1
• UniProt Accession #: P15337
• Molecular Weight: 35,081 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 1 isoform B
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: Creb1
• NCBI Official Synonym Symbols: Creb
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: CREB-binding protein
• UniProt Gene Name: Creb1
• UniProt Synonym Gene Names: Creb-1; CREB-1; cAMP-responsive element-binding protein 1
• UniProt Entry Name: CREB1_RAT

NCBI Description

binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; mitochondrion; nuclear chromatin; chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor binding; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; lactation; response to glucagon stimulus; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; positive regulation of multicellular organism growth; protein amino acid phosphorylation; response to organic substance; regulation of apoptosis; regulation of transcription, DNA-dependent; mammary gland development; positive regulation of transforming growth factor-beta3 production; transforming growth factor beta receptor signaling pathway; cell differentiation; positive regulation of transcriptional preinitiation complex assembly; response to drug; protein stabilization; secretory granule organization and biogenesis; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; regulation of circadian rhythm; memory; axonogenesis; pituitary gland development; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell size