Western Blot (WB)
(Cxxc4 antibody (pAb) tested by Western blot. Detection of Cxxc4 antibody by Western blot analysis. Lane 1: Mouse ES cytoplasmic extract. Lane 2: F9 cytoplasmic extract. Lane 3: P19 cytoplasmic extract. Lane 4: HEK293 whole cell extract. 30 ug of lysate per lane probed with Cxxc4 antibody at a dilution of 1:1,000.)
Rabbit anti-Human, Mouse Cxxc4 Polyclonal Antibody | anti-Cxxc4 antibody
Cxxc4 antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Cxxc4; Polyclonal Antibody; Cxxc4 antibody (pAb); Idax; 9330210J02Rik; C030003J12Rik; anti-Cxxc4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Cxxc4 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:1,000-1:2,500 dilution
The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
WB: 1:1,000-1:2,500 dilution
The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of mouse Cxxc4.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Cxxc4 antibody (pAb) tested by Western blot. Detection of Cxxc4 antibody by Western blot analysis. Lane 1: Mouse ES cytoplasmic extract. Lane 2: F9 cytoplasmic extract. Lane 3: P19 cytoplasmic extract. Lane 4: HEK293 whole cell extract. 30 ug of lysate per lane probed with Cxxc4 antibody at a dilution of 1:1,000.)
Western Blot (WB)
(Cxxc4 antibody (pAb) tested by Western blot. Detection of Cxxc4 antibody by Western blot analysis. Lane 1: Mouse ES cytoplasmic extract. Lane 2: F9 cytoplasmic extract. Lane 3: P19 cytoplasmic extract. Lane 4: HEK293 whole cell extract. 30 ug of lysate per lane probed with Cxxc4 antibody at a dilution of 1:1,000.)
Related Product Information for anti-Cxxc4 antibody
Short Description: Our Cxxc4 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human and Mouse samples.
Background: Cxxc4 (CXXC Finger Protein 4) acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1 and has been implicated in malignant renal cell carcinoma and colonic villous adenoma. Recently, Cxxc4 has been shown to be involved in the regulation of DNA demethylation. It binds to unmethylated CpGs in DNA and is involved in TET2 downregulation.
Background: Cxxc4 (CXXC Finger Protein 4) acts as a negative regulator of the Wnt signaling pathway via its interaction with DVL1 and has been implicated in malignant renal cell carcinoma and colonic villous adenoma. Recently, Cxxc4 has been shown to be involved in the regulation of DNA demethylation. It binds to unmethylated CpGs in DNA and is involved in TET2 downregulation.
Product Categories/Family for anti-Cxxc4 antibody
NCBI and Uniprot Product Information
Molecular Weight
42kDa
Protein Family
Similar Products
Product Notes
The Cxxc4 (Catalog #AAA389007) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cxxc4 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cxxc4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:1,000-1:2,500 dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the Cxxc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cxxc4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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